Deconvoluting nitric oxide-protein interactions with spatially resolved multiplex imaging
文献类型：期刊论文
| 作者 | Li, Yi5; Pan, Kaijun4; Gao, Yanan4; Li, Jia1,4 ; Zang, Yi1,3; Li, Xin2,5
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| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2024-04-08 |
| 页码 | 10 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/d4sc00767k |
| 通讯作者 | Zang, Yi(yzang@lglab.ac.cn) ; Li, Xin(lixin81@zju.edu.cn) |
| 英文摘要 | Simultaneous imaging of nitric oxide (NO) and its proximal proteins should facilitate the deconvolution of NO-protein interactions. While immunostaining is a primary assay to localize proteins in non-genetically manipulated samples, NO imaging probes with immunostaining-compatible signals remain unexplored. Herein, probe NOP-1 was developed with an NO-triggered proximal protein labeling capacity and fluorogenic signals. The trick is to fuse the native chemical ligation of acyl benzotriazole with the protein-conjugation-induced fluorogenic response of Si-rhodamine fluorophore. NOP-1 predominantly existed in the non-fluorescent spirocyclic form. Yet, its acyl o-phenylenediamine moiety was readily activated by NO into acyl benzotriazole to conjugate proximal proteins, providing a fluorogenic response and translating the transient cellular NO signal into a permanent stain compatible with immunostaining. NOP-1 was utilized to investigate NO signaling in hypoglycemia-induced neurological injury, providing direct evidence of NO-induced apoptosis during hypoglycemia. Mechanistically, multiplex imaging revealed the overlap of cellular NOP-1 fluorescence with immunofluorescence for alpha-tubulin and NO2-Tyr. Importantly, alpha-tubulin was resolved from NOP-1 labeled proteins. These results suggest that NO played a role in hypoglycemia-induced apoptosis, at least in part, through nitrating alpha-tubulin. This study fills a crucial gap in current imaging probes, providing a valuable tool for unraveling the complexities of NO signaling in biological processes. Simultaneous imaging of nitric oxide (NO) and its proximal proteins should facilitate the deconvolution of NO-protein interactions. |
| WOS关键词 | FLUOROGENIC PROBES ; ALPHA-TUBULIN ; SYNTHASE ; HYPOGLYCEMIA ; DYSFUNCTION ; SYSTEM ; BRAIN ; CELLS |
| 资助项目 | Natural Science Foundation of Zhejiang Province[22077112] ; Natural Science Foundation of Zhejiang Province[22377106] ; Natural Science Foundation of Zhejiang Province[82151219] ; National Natural Science Foundations of China[LZ23H300001] ; National Natural Science Foundations of Zhejiang province[22ZR1415200] ; Natural Science Foundation of Shanghai[2021] ; National Program for Support of Top-notch Young Professionals[23XD1450800] ; Program of Shanghai Academic Research Leader |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001198144100001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310553]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zang, Yi; Li, Xin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 2.Zhejiang Univ, Innovat Ctr Yangtze River Delta, Future Hlth Lab, Jiashan 314100, Peoples R China 3.Lingang Lab, Shanghai 201203, Peoples R China 4.China Pharmaceut Univ, Jiangsu Key Lab Drug Screening, Nanjing 210009, Peoples R China 5.Zhejiang Univ, Coll Pharmaceut Sci, Natl Key Lab Adv Drug Delivery & Release Syst, 866 Yuhangtang Rd, Hangzhou 310058, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yi,Pan, Kaijun,Gao, Yanan,et al. Deconvoluting nitric oxide-protein interactions with spatially resolved multiplex imaging[J]. CHEMICAL SCIENCE,2024:10. |
| APA | Li, Yi,Pan, Kaijun,Gao, Yanan,Li, Jia,Zang, Yi,&Li, Xin.(2024).Deconvoluting nitric oxide-protein interactions with spatially resolved multiplex imaging.CHEMICAL SCIENCE,10. |
| MLA | Li, Yi,et al."Deconvoluting nitric oxide-protein interactions with spatially resolved multiplex imaging".CHEMICAL SCIENCE (2024):10. |
入库方式： OAI收割
来源：上海药物研究所
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