Enhancing Specific Fluorescence In Situ Hybridization with Quantum Dots for Single-Molecule RNA Imaging in Formalin-Fixed Paraffin-Embedded Tumor Tissues
文献类型：期刊论文
| 作者 | Zhao, Zeyin4,5; Jiang, Mengyuan3,4; He, Chen4; Yin, Wenjuan4; Feng, Yawei4,5; Wang, Peng1,2; Ying, Lisha4; Fu, Ting4,5; Su, Dan4; Peng, Ruizi4,5 |
| 刊名 | ACS NANO
 |
| 出版日期 | 2024-03-28 |
| 卷号 | 18期号:14页码:9958-9968 |
| 关键词 | single molecule split-FISH quantum dots RNA imaging quantification FFPE clinical samples diagnostic pathology |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.3c10216 |
| 通讯作者 | Su, Dan(sudan@zjcc.org.cn) ; Peng, Ruizi(r_peng@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn) |
| 英文摘要 | Single-molecule fluorescence in situ hybridization (smFISH) represents a promising approach for the quantitative analysis of nucleic acid biomarkers in clinical tissue samples. However, low signal intensity and high background noise are complications that arise from diagnostic pathology when performed with smFISH-based RNA imaging in formalin-fixed paraffin-embedded (FFPE) tissue specimens. Moreover, the associated complex procedures can produce uncertain results and poor image quality. Herein, by combining the high specificity of split DNA probes with the high signal readout of ZnCdSe/ZnS quantum dot (QD) labeling, we introduce QD split-FISH, a high-brightness smFISH technology, to quantify the expression of mRNA in both cell lines and clinical FFPE tissue samples of breast cancer and lung squamous carcinoma. Owing to its high signal-to-noise ratio, QD split-FISH is a fast, inexpensive, and sensitive method for quantifying mRNA expression in FFPE tumor tissues, making it suitable for biomarker imaging and diagnostic pathology. |
| WOS关键词 | AMPLIFICATION ; PROTEIN ; POLYMERASE ; BIOMARKERS ; PROBES ; CELLS |
| 资助项目 | National Natural Science Foundation of China ; Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences[T2188102] ; Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences[22104035] ; National Natural Science Foundation of China[YXD24B0403] ; Zhejiang Provincial Natural Science Foundation of China[2022JJ40039] ; Hunan Provincial Natural Science Foundation of China[2022R01006] ; Zhejiang Leading Innovation and Entrepreneurship Team |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001194378800001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310556]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Su, Dan; Peng, Ruizi; Tan, Weihong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Materia Med, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Mol Med, Hangzhou 310024, Zhejiang, Peoples R China 3.Univ Sci & Technol China, Sch Chem & Mat Sci, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Zhejiang Canc Hosp, Hangzhou Inst Med HIM, Dept Pathol,Key Lab Zhejiang Prov Aptamers & Thera, Hangzhou 310022, Zhejiang, Peoples R China 5.Hunan Univ, Coll Chem & Chem Engn, Aptamer Engn Ctr Hunan Prov, Coll Biol,Mol Sci & Biomed Lab MBL,State Key Lab C, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zeyin,Jiang, Mengyuan,He, Chen,et al. Enhancing Specific Fluorescence In Situ Hybridization with Quantum Dots for Single-Molecule RNA Imaging in Formalin-Fixed Paraffin-Embedded Tumor Tissues[J]. ACS NANO,2024,18(14):9958-9968. |
| APA | Zhao, Zeyin.,Jiang, Mengyuan.,He, Chen.,Yin, Wenjuan.,Feng, Yawei.,...&Tan, Weihong.(2024).Enhancing Specific Fluorescence In Situ Hybridization with Quantum Dots for Single-Molecule RNA Imaging in Formalin-Fixed Paraffin-Embedded Tumor Tissues.ACS NANO,18(14),9958-9968. |
| MLA | Zhao, Zeyin,et al."Enhancing Specific Fluorescence In Situ Hybridization with Quantum Dots for Single-Molecule RNA Imaging in Formalin-Fixed Paraffin-Embedded Tumor Tissues".ACS NANO 18.14(2024):9958-9968. |
入库方式： OAI收割
来源：上海药物研究所
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