Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
文献类型：期刊论文
| 作者 | Li, Yanan4; Chen, Zongfu4; Zhang, Haibin3 ; Liu, Ruoyu2; Chen, Shuichun1; Lin, Li4
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| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2024-03-06 |
| 页码 | 12 |
| 关键词 | Holothuroidea environmental adaptation positive selection point mutation |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-023-3106-5 |
| 英文摘要 | Manganese superoxide dismutase (MnSOD) is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria. In a dark, high-pressure, and low-temperature deep-sea environment, MnSOD is essential for the survival of sea cucumbers. Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers. To explore their environmental adaptation mechanism, we conducted environmental selection pressure analysis through the branching site model of PAML software. We obtained night positive selection sites, and two of them were significant (97F -> H, 134K -> V): 97F -> H located in a highly conservative characteristic sequence, and its polarity change might have a great impact on the function of MnSOD; 134K -> V had a change in piezophilic ability, which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea. To further study the effect of these two positive selection sites on MnSOD, we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL. Results show that 97F -> H, 134K -> V might improve MnSOD's efficiency of scavenging superoxide anion and its ability to resist high hydrostatic pressure by moderately reducing its stability. The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity, piezophilic behavior, and local stability. This study revealed the correlation between MnSOD and extreme environment, and will help improve our understanding of the organism's adaptation mechanisms in deep sea. |
| WOS关键词 | PHYLOGENETIC ANALYSIS ; MUTATIONS ; COPPER |
| 资助项目 | Guangdong Province Basic and Applied Basic Research Fund Project[2020A1515110826] ; National Natural Science Foundation of China[42006115] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2021036] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001178950400001 |
| 出版者 | SCIENCE PRESS |
| 资助机构 | Guangdong Province Basic and Applied Basic Research Fund Project ; National Natural Science Foundation of China ; Major Scientific and Technological Projects of Hainan Province |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/10931]  |
| 专题 | 深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组 |
| 通讯作者 | Lin, Li |
| 作者单位 | 1.Zhanjiang Tianli Sea Cucumber New Technol Dev Co L, Zhanjiang 524000, Peoples R China 2.Fujian Agr & Forestry Univ, Coll Marine Sci, Fuzhou 350002, Peoples R China 3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yanan,Chen, Zongfu,Zhang, Haibin,et al. Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:12. |
| APA | Li, Yanan,Chen, Zongfu,Zhang, Haibin,Liu, Ruoyu,Chen, Shuichun,&Lin, Li.(2024).Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,12. |
| MLA | Li, Yanan,et al."Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):12. |
入库方式： OAI收割
来源：深海科学与工程研究所
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