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Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803

文献类型：期刊论文

作者Zhang, Yumeng1,2,3; Wang, Yaqi1,2,3; Wei, Wei1,2,3; Wang, Min4; Jia, Shuzhao4; Yang, Mingkun1,2,3; Ge, Feng1,2,3
刊名FRONTIERS IN PLANT SCIENCE
出版日期2022-09-29
卷号13页码:16
关键词cyanobacteria proteases proteostasis quantitative proteomics ClpX
ISSN号1664-462X
DOI10.3389/fpls.2022.994056
通讯作者Yang, Mingkun(yangmingkun@ihb.ac.cn) ; Ge, Feng(gefeng@ihb.ac.cn)
英文摘要Protein homeostasis is tightly regulated by protein quality control systems such as chaperones and proteases. In cyanobacteria, the ClpXP proteolytic complex is regarded as a representative proteolytic system and consists of a hexameric ATPase ClpX and a tetradecameric peptidase ClpP. However, the functions and molecular mechanisms of ClpX in cyanobacteria remain unclear. This study aimed to decipher the unique contributions and regulatory networks of ClpX in the model cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We showed that the interruption of clpX led to slower growth, decreased high light tolerance, and impaired photosynthetic cyclic electron transfer. A quantitative proteomic strategy was employed to globally identify ClpX-regulated proteins in Synechocystis cells. In total, we identified 172 differentially expressed proteins (DEPs) upon the interruption of clpX. Functional analysis revealed that these DEPs are involved in diverse biological processes, including glycolysis, nitrogen assimilation, photosynthetic electron transport, ATP-binding cassette (ABC) transporters, and two-component signal transduction. The expression of 24 DEPs was confirmed by parallel reaction monitoring (PRM) analysis. In particular, many hypothetical or unknown proteins were found to be regulated by ClpX, providing new candidates for future functional studies on ClpX. Together, our study provides a comprehensive ClpX-regulated protein network, and the results serve as an important resource for understanding protein quality control systems in cyanobacteria.
WOS关键词SP PCC-6803 ; PROTEASE ; SYSTEM ; DEGRADATION ; PROTEOLYSIS ; RESISTANCE ; REVEALS ; STRESS ; IDENTIFICATION ; POLYPHOSPHATE
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000868254800001
资助机构National Key Research and Development Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; China Postdoctoral Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology
源URL[http://ir.yic.ac.cn/handle/133337/34808]  
专题中国科学院烟台海岸带研究所
通讯作者Yang, Mingkun; Ge, Feng
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yumeng,Wang, Yaqi,Wei, Wei,et al. Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803[J]. FRONTIERS IN PLANT SCIENCE,2022,13:16.
APA Zhang, Yumeng.,Wang, Yaqi.,Wei, Wei.,Wang, Min.,Jia, Shuzhao.,...&Ge, Feng.(2022).Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803.FRONTIERS IN PLANT SCIENCE,13,16.
MLA Zhang, Yumeng,et al."Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803".FRONTIERS IN PLANT SCIENCE 13(2022):16.

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来源：烟台海岸带研究所

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