Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions
文献类型：期刊论文
| 作者 | Ding, Hao1; Zhang, Mingdi1; Wang, Xiaochun1; He, Shuai2; Wang, Xiaokun1; Chen, Lingxin3,4,5
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2024-04-22 |
| 卷号 | 1299页码:10 |
| 关键词 | Ochratoxin A Dual-channel Signal on In-system correction Mini-program |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2024.342440 |
| 通讯作者 | Wang, Xiaokun(wxkun87@126.com) |
| 英文摘要 | Ochratoxin A (OTA) is a hazardous food contaminant with significant health risks. Dual -channel OTA detection is noted for its cross-reference capability and high accuracy. Still, challenges in addressing in -system corrections and "signal off" related false positives and limited signal gains remain. Herein, we developed a dual -channel "signal on" aptasensor with one recognition process and two independent signal outputs for OTA analysis. The OTA aptamer binds to magnetic beads (MBs) and partially hybridizes with a complementary -trigger (cDNATrigger) sequence. Adding OTA disrupts the duplex sequence, leading to G-quadruplex (G4) formation and enrichment on the MBs, which then interacts with hemin to catalyze a color signal. Concurrently, the freed cDNA-Trigger catalyzes an enzyme -free DNA circuit, producing a fluorescence signal. The magnetic enrichment and signal amplification strategies make the proposed assay demonstrate excellent sensitivity toward OTA, with limits of detection (LOD) of 0.017 pM in the fluorescence channel and 48.1 pM in the colorimetric channel. Both channels have effectively detected OTA in grape juice and baijiu, demonstrating their applicability and reliability. Moreover, given the widespread use of smartphones globally, a mini -program with a self -correction function was designed to facilitate on -site colorimetric channel monitoring, making OTA detection more accessible and user-friendly. |
| WOS关键词 | ELISA ; APTASENSOR ; ASSAY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001209090700001 |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/35351]  |
| 专题 | 中国科学院烟台海岸带研究所 |
| 通讯作者 | Wang, Xiaokun |
| 作者单位 | 1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Univ New South Wales, Sch Mech & Mfg Engn, Sydney 2052, Australia 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 5.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Hao,Zhang, Mingdi,Wang, Xiaochun,et al. Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions[J]. ANALYTICA CHIMICA ACTA,2024,1299:10. |
| APA | Ding, Hao,Zhang, Mingdi,Wang, Xiaochun,He, Shuai,Wang, Xiaokun,&Chen, Lingxin.(2024).Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions.ANALYTICA CHIMICA ACTA,1299,10. |
| MLA | Ding, Hao,et al."Colorimetric and fluorescent independent dual "signal on" biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions".ANALYTICA CHIMICA ACTA 1299(2024):10. |
入库方式： OAI收割
来源：烟台海岸带研究所
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