Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system
文献类型：期刊论文
| 作者 | Li, Yingqi3,4,5; Duan, Jinjin5; Li, You5; Zhang, Meihui5; Wu, Jiaan5; Wang, Guanhao5; Li, Shuanqing5; Hu, Zhangsen5; Qu, Yi5; Li, Yunhe5 |
| 刊名 | STEM CELL RESEARCH & THERAPY
 |
| 出版日期 | 2024-04-19 |
| 卷号 | 15期号:1页码:16 |
| 关键词 | Serotonin neurons FEV-EGFP reporter Human pluripotent stem cells Differentiation CRISPR/Cas9 |
| DOI | 10.1186/s13287-024-03728-x |
| 通讯作者 | Cao, Lining(crystalvivining@aliyun.com) ; Lu, Jianfeng(Lu.jianfeng@tongji.edu.cn) |
| 英文摘要 | Background The detailed transcriptomic profiles during human serotonin neuron (SN) differentiation remain elusive. The establishment of a reporter system based on SN terminal selector holds promise to produce highly-purified cells with an early serotonergic fate and help elucidate the molecular events during human SN development process.Methods A fifth Ewing variant (FEV)-EGFP reporter system was established by CRISPR/Cas9 technology to indicate SN since postmitotic stage. FACS was performed to purify SN from the heterogeneous cell populations. RNA-sequencing analysis was performed for cells at four key stages of differentiation (pluripotent stem cells, serotonergic neural progenitors, purified postmitotic SN and purifed mature SN) to explore the transcriptomic dynamics during SN differentiation.Results We found that human serotonergic fate specification may commence as early as day 21 of differentiation from human pluripotent stem cells. Furthermore, the transcriptional factors ZIC1, HOXA2 and MSX2 were identified as the hub genes responsible for orchestrating serotonergic fate determination.Conclusions For the first time, we exposed the developmental transcriptomic profiles of human SN via FEV reporter system, which will further our understanding for the development process of human SN. |
| WOS关键词 | G-PROTEIN GAMMA(3) ; GENERATION ; SURVIVAL ; PET-1 ; MICE |
| 资助项目 | National Natural Science Foundation of China |
| WOS研究方向 | Cell Biology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001205529400001 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310650]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Cao, Lining; Lu, Jianfeng |
| 作者单位 | 1.Tongji Univ, Suzhou Inst, Suzhou, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Beijing, Peoples R China 5.Tongji Univ, Shanghai YangZhi Rehabil Hosp, Shanghai Sunshine Rehabil Ctr, Frontier Sci Ctr Stem Cell Res,Sch Life Sci & Tech, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yingqi,Duan, Jinjin,Li, You,et al. Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system[J]. STEM CELL RESEARCH & THERAPY,2024,15(1):16. |
| APA | Li, Yingqi.,Duan, Jinjin.,Li, You.,Zhang, Meihui.,Wu, Jiaan.,...&Lu, Jianfeng.(2024).Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system.STEM CELL RESEARCH & THERAPY,15(1),16. |
| MLA | Li, Yingqi,et al."Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system".STEM CELL RESEARCH & THERAPY 15.1(2024):16. |
入库方式： OAI收割
来源：上海药物研究所
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