Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore
文献类型：期刊论文
| 作者 | Yao, Guangda5,6,7; Tian, Yinping4; Ke, Wenjun3,7; Fang, Jie7; Ma, Shengzhou4; Li, Tiehai3,4; Cheng, Xi2,7; Xia, Bingqing3,7; Wen, Liuqing3,4; Gao, Zhaobing1,3,7
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| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
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| 出版日期 | 2024-04-11 |
| 页码 | 11 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.4c02081 |
| 通讯作者 | Cheng, Xi(xicheng@simm.ac.cn) ; Xia, Bingqing(xiabingqing@simm.ac.cn) ; Wen, Liuqing(lwen@simm.ac.cn) ; Gao, Zhaobing(zbgao@simm.ac.cn) |
| 英文摘要 | The crucial roles that glycans play in biological systems are determined by their structures. However, the analysis of glycan structures still has numerous bottlenecks due to their inherent complexities. The nanopore technology has emerged as a powerful sensor for DNA sequencing and peptide detection. This has a significant impact on the development of a related research area. Currently, nanopores are beginning to be applied for the detection of simple glycans, but the analysis of complex glycans by this technology is still challenging. Here, we designed an engineered alpha-hemolysin nanopore M113R/T115A to achieve the sensing of complex glycans at micromolar concentrations and under label-free conditions. By extracting characteristic features to depict a three-dimensional (3D) scatter plot, glycans with different numbers of functional groups, various chain lengths ranging from disaccharide to decasaccharide, and distinct glycosidic linkages could be distinguished. Molecular dynamics (MD) simulations show different behaviors of glycans with beta 1,3- or beta 1,4-glycosidic bonds in nanopores. More importantly, the designed nanopore system permitted the discrimination of each glycan isomer with different lengths in a mixture with a separation ratio of over 0.9. This work represents a proof-of-concept demonstration that complex glycans can be analyzed using nanopore sequencing technology. |
| 资助项目 | Natural Science Foundation of Shanghai Municipality ; Shanghai Municipal Science and Technology Major Project[81825021] ; National Science Fund of Distinguished Young Scholars[2019285] ; National Science Fund of Distinguished Young Scholars[2021333] ; National Science Fund of Distinguished Young Scholars[2022077] ; Fund of Youth Innovation Promotion Association[92169202] ; Fund of Youth Innovation Promotion Association[82341058] ; National Natural Science Foundation of China[20ZR1474200] ; Fund of Shanghai Science and Technology Innovation Action Plan[22QA1411000] ; Shanghai Rising-Star Program[22ZR1474000] ; Natural Science Foundation of Shanghai[LG202101-01-04] ; Natural Science Foundation of Shanghai[LG202102-01-01] ; National Key Laboratory Program of China[2020YFC0842000] ; National Key Research and Development Program of China[XDA12050308] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001201289100001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310765]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Cheng, Xi; Xia, Bingqing; Wen, Liuqing; Gao, Zhaobing |
| 作者单位 | 1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 2.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 330106, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, Shanghai 201203, Peoples R China 5.Lingang Lab, Shanghai 200031, Peoples R China 6.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 7.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Guangda,Tian, Yinping,Ke, Wenjun,et al. Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2024:11. |
| APA | Yao, Guangda.,Tian, Yinping.,Ke, Wenjun.,Fang, Jie.,Ma, Shengzhou.,...&Gao, Zhaobing.(2024).Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,11. |
| MLA | Yao, Guangda,et al."Direct Identification of Complex Glycans via a Highly Sensitive Engineered Nanopore".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2024):11. |
入库方式： OAI收割
来源：上海药物研究所
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