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Nanopore-based glycan sequencing: state of the art and future prospects

文献类型：期刊论文

作者Yao, Guangda3,4,5; Ke, Wenjun2,5; Xia, Bingqing2,5; Gao, Zhaobing1,2,5
刊名CHEMICAL SCIENCE
出版日期2024-05-01
卷号15期号:17页码:6229-6243
ISSN号2041-6520
DOI10.1039/d4sc01466a
通讯作者Xia, Bingqing(xiabingqing@simm.ac.cn) ; Gao, Zhaobing(bgao@simm.ac.cn)
英文摘要Sequencing of biomacromolecules is a crucial cornerstone in life sciences. Glycans, one of the fundamental biomolecules, derive their physiological and pathological functions from their structures. Glycan sequencing faces challenges due to its structural complexity and current detection technology limitations. As a highly sensitive sensor, nanopores can directly convert nucleic acid sequence information into electrical signals, spearheading the revolution of third-generation nucleic acid sequencing technologies. However, their potential for deciphering complex glycans remains untapped. Initial attempts demonstrated the significant sensitivity of nanopores in glycan sensing, which provided the theoretical basis and insights for the realization of nanopore-based glycan sequencing. Here, we present three potential technical routes to employ nanopore technology in glycan sequencing for the first time. The three novel technical routes include: strand sequencing, capturing glycan chains as they translocate through nanopores; sequential hydrolysis sequencing, capturing released monosaccharides one by one; splicing sequencing, mapping signals from hydrolyzed glycan fragments to an oligosaccharide database/library. Designing suitable nanopores, enzymes, and motors, and extracting characteristic signals pose major challenges, potentially aided by artificial intelligence. It would be highly desirable to design an all-in-one high-throughput glycan sequencer instrument by integrating a sample processing unit, nanopore array, and signal acquisition system into a microfluidic device. The nanopore sequencer invention calls for intensive multidisciplinary cooperation including electrochemistry, glycochemistry, engineering, materials, enzymology, etc. Advancing glycan sequencing will promote the development of basic research and facilitate the discovery of glycan-based drugs and disease markers, fostering progress in glycoscience and even life sciences. This perspective outlines three potential routes for nanopore-based glycan sequencing, highlighting their potential applications and offering insights to meet associated challenges. It also introduces the concept of nanopore-based glycan sequencer.
WOS关键词ALPHA-HEMOLYSIN ; OUTER-MEMBRANE ; MASS-SPECTROMETRY ; ESCHERICHIA-COLI ; PORE FORMATION ; CELL BIOLOGY ; N-GLYCANS ; PROTEIN ; DNA ; OLIGOSACCHARIDES
资助项目National Natural Science Foundation of China ; Shanghai Municipal Science and Technology Major Project[81825021] ; National Science Fund of Distinguished Young Scholars[2019285] ; National Science Fund of Distinguished Young Scholars[2021333] ; National Science Fund of Distinguished Young Scholars[2022077] ; Fund of Youth Innovation Promotion Association[92169202] ; Fund of Youth Innovation Promotion Association[82341058] ; National Natural Science Foundation of China[20ZR1474200] ; Fund of Shanghai Science and Technology Innovation Action Plan[22QA1411000] ; Shanghai Rising-Star Program[23JC1404301] ; Science and Technology Commission of Shanghai Municipality[22ZR1474000] ; Natural Science Foundation of Shanghai[LG202101-01-04] ; Natural Science Foundation of Shanghai[LG202102-01-01] ; National Key Laboratory Program of China[2020YFC0842000] ; National Key Research and Development Program of China[XDA12050308] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001198678900001
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.183/handle/2S10ELR8/310768]  
专题新药研究国家重点实验室
通讯作者Xia, Bingqing; Gao, Zhaobing
作者单位1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Lingang Lab, Shanghai 200031, Peoples R China
4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Yao, Guangda,Ke, Wenjun,Xia, Bingqing,et al. Nanopore-based glycan sequencing: state of the art and future prospects[J]. CHEMICAL SCIENCE,2024,15(17):6229-6243.
APA Yao, Guangda,Ke, Wenjun,Xia, Bingqing,&Gao, Zhaobing.(2024).Nanopore-based glycan sequencing: state of the art and future prospects.CHEMICAL SCIENCE,15(17),6229-6243.
MLA Yao, Guangda,et al."Nanopore-based glycan sequencing: state of the art and future prospects".CHEMICAL SCIENCE 15.17(2024):6229-6243.

入库方式： OAI收割

来源：上海药物研究所

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