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Evolutionary ecology of denitrifying methanotrophic NC10 bacteria in the deep-sea biosphere

文献类型：期刊论文

作者Li, Yingdong2,3,4; Wang, Ting1,4; Jing, Hongmei2,3,4; Xiao, Yao1,4
刊名MOLECULAR ECOLOGY
出版日期2024-05-06
页码16
关键词deep-sea biosphere evolutionary ecology NC10 phylum n-DAMO
ISSN号0962-1083
DOI10.1111/mec.17372
英文摘要The NC10 phylum links anaerobic methane oxidation to nitrite denitrification through a unique O2-producing intra-aerobic methanotrophic pathway. Although numerous amplicon-based studies revealed the distribution of this phylum, comprehensive genomic insights and niche characterization in deep-sea environments were still largely unknown. In this study, we extensively surveyed the NC10 bacteria across diverse deep-sea environments, including waters, sediments, cold seeps, biofilms, rocky substrates, and subseafloor aquifers. We then reconstructed and analysed 38 metagenome-assembled genomes (MAGs), and revealed the extensive distribution of NC10 bacteria and their intense selective pressure in these harsh environments. Isotopic analyses combined with gene expression profiling confirmed that active nitrite-dependent anaerobic methane oxidation (n-DAMO) occurs within deep-sea sediments. In addition, the identification of the Wood-Ljungdahl (WL) and 3-hydroxypropionate/4-hydroxybutyrat (3HB/4HP) pathways in these MAGs suggests their capability for carbon fixation as chemoautotrophs in these deep-sea environments. Indeed, we found that for their survival in the oligotrophic deep-sea biosphere, NC10 bacteria encode two branches of the WL pathway, utilizing acetyl-CoA from the carbonyl branch for citric acid cycle-based energy production and methane from the methyl branch for n-DAMO. The observed low ratios of non-synonymous substitutions to synonymous substitutions (pN/pS) in n-DAMO-related genes across these habitats suggest a pronounced purifying selection that is critical for the survival of NC10 bacteria in oligotrophic deep-sea environments. These findings not only advance our understanding of the evolutionary adaptations of NC10 bacteria but also underscore the intricate coupling between the carbon and nitrogen cycles within deep-sea ecosystems, driven by this bacterial phylum.
WOS关键词ANAEROBIC METHANE OXIDATION ; ALIGNMENT ; NITRITE ; SEDIMENTS ; OXYGEN ; ENRICHMENT ; DIVERSITY ; GENOMES ; PROTEIN ; DRIVEN
资助项目Innovational Found for the scientific and technological personnel of Hainan Province[KJRC2023C37] ; National Key R&D Program of China[2022YFC2805505] ; Hainan Province Science and Technology special fund[ZDKJ2021036] ; Special Research Assistant Grant of Chinese academy of science[E4710101] ; Hainan Province Postdoctoral Research Project[E33D0101]
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
语种英语
WOS记录号WOS:001214290900001
出版者WILEY
资助机构Innovational Found for the scientific and technological personnel of Hainan Province ; National Key R&D Program of China ; Hainan Province Science and Technology special fund ; Special Research Assistant Grant of Chinese academy of science ; Hainan Province Postdoctoral Research Project
源URL[http://ir.idsse.ac.cn/handle/183446/11019]  
专题深海科学研究部_深海生物学研究室_海洋微生物分子生态学研究组
研究生部
通讯作者Jing, Hongmei
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, HKUST CAS Sanya Joint Lab Marine Sci Res, Sanya, Peoples R China
3.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, CAS Key Lab Expt Study Deep Sea Extreme Condit, Sanya, Peoples R China
推荐引用方式
GB/T 7714		 Li, Yingdong,Wang, Ting,Jing, Hongmei,et al. Evolutionary ecology of denitrifying methanotrophic NC10 bacteria in the deep-sea biosphere[J]. MOLECULAR ECOLOGY,2024:16.
APA Li, Yingdong,Wang, Ting,Jing, Hongmei,&Xiao, Yao.(2024).Evolutionary ecology of denitrifying methanotrophic NC10 bacteria in the deep-sea biosphere.MOLECULAR ECOLOGY,16.
MLA Li, Yingdong,et al."Evolutionary ecology of denitrifying methanotrophic NC10 bacteria in the deep-sea biosphere".MOLECULAR ECOLOGY (2024):16.

入库方式： OAI收割

来源：深海科学与工程研究所

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