Apparent isotope effects of reversible enzymatic reactions
文献类型：期刊论文
| 作者 | He YY(何雨旸); Thullner, Martin; Qin, Rui; Pearson, Ann |
| 刊名 | GEOCHIMICA ET COSMOCHIMICA ACTA
 |
| 出版日期 | 2024-04-01 |
| 卷号 | 370页码:188-198 |
| 关键词 | Rayleigh Distillation Model Isotope fractionation factor Reverse Michaelis-Menten kinetics Bonded isotope effect Batch experiment |
| ISSN号 | 0016-7037 |
| DOI | 10.1016/j.gca.2024.01.021 |
| 通讯作者 | He, Yuyang(yhe@mail.iggcas.ac.cn) |
| 英文摘要 | Accurately predicting apparent isotope effects of biogeochemical processes can aid the interpretation of laboratory batch experiments and field observations. It has been observed that the apparent instantaneous isotope fractionation factor changes with residual substrate concentration during a reaction process. The change can be attributed to the reversibility of a biogeochemical reaction, which is often overlooked for convenience. Here, we explore the influence of reverse reactions on high -dimensional isotope effects in the framework of Reverse Michaelis-Menten (RMM) kinetics. The apparent instantaneous isotope fractionation factors, as well as the isotope fractionation relationships between two elements of the same nonlabile compound in the RMM model, are functions of specific affinities of the product and substrate to an enzyme and the product -substrate ratios for both isotopes. In our cases, the influence of reaction reversibility is negligible when the substrate consumption rate is less than 63%. The analysis of the simultaneous stable isotope fractionation of two elements in a compound and of the relationship between the associated isotope fractionation factors can serve as a more effective indicator for characterizing the reaction mechanism. |
| 分类号 | 一类 |
| WOS关键词 | DISSIMILATORY SULFATE REDUCTION ; BIOAVAILABILITY RESTRICTIONS ; NITROGEN LIMITATION ; NITRATE REDUCTION ; FRACTIONATION ; SULFUR ; OXYGEN ; KINETICS ; DENITRIFICATION ; EXCHANGE |
| 资助项目 | National Science Foundation of China (NSFC)[42150202] ; National Science Foundation of China (NSFC)[42173041] ; Key Research Program of the Institute of Geology and Geophysics, Chinese Academy of Sciences[IGGCAS-202204] |
| WOS研究方向 | Geochemistry & Geophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:001223315400001 |
| 资助机构 | National Science Foundation of China (NSFC) ; Key Research Program of the Institute of Geology and Geophysics, Chinese Academy of Sciences |
| 其他责任者 | He, Yuyang |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/95259]  |
| 专题 | 力学研究所_高温气体动力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | He YY,Thullner, Martin,Qin, Rui,et al. Apparent isotope effects of reversible enzymatic reactions[J]. GEOCHIMICA ET COSMOCHIMICA ACTA,2024,370:188-198. |
| APA | 何雨旸,Thullner, Martin,Qin, Rui,&Pearson, Ann.(2024).Apparent isotope effects of reversible enzymatic reactions.GEOCHIMICA ET COSMOCHIMICA ACTA,370,188-198. |
| MLA | 何雨旸,et al."Apparent isotope effects of reversible enzymatic reactions".GEOCHIMICA ET COSMOCHIMICA ACTA 370(2024):188-198. |
入库方式： OAI收割
来源：力学研究所
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