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Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow

文献类型：期刊论文

作者Chen, Xueping8; Fan, Ke8; Lu, Jun2,8; Zhang, Sheng8; Dong, Jianhua8; Qin, Jisheng8; Fan, Weihua8; Wang, Yan8; Zhang, Yiyuan8; Peng, Huo8
刊名RESEARCH
出版日期2024-03-07
卷号7页码:16
ISSN号2096-5168
DOI10.34133/research.0338
通讯作者Zhang, Xiao(zhang_xiao@gibh.ac.cn)
英文摘要Somatic cell reprogramming generates induced pluripotent stem cells (iPSCs), which serve as a crucial source of seed cells for personalized disease modeling and treatment in regenerative medicine. However, the process of reprogramming often causes substantial lineage manipulations, thereby increasing cellular heterogeneity. As a consequence, the process of harvesting monoclonal iPSCs is labor-intensive and leads to decreased reproducibility. Here, we report the first in-house developed robotic platform that uses a pin-tip-based micro-structure to manipulate radial shear flow for automated monoclonal iPSC colony selection (similar to 1 s) in a non-invasive and label-free manner, which includes tasks for somatic cell reprogramming culturing, medium changes; time-lapse-based high-content imaging; and iPSCs monoclonal colony detection, selection, and expansion. Throughput-wise, this automated robotic system can perform approximately 24 somatic cell reprogramming tasks within 50 days in parallel via a scheduling program. Moreover, thanks to a dual flow-based iPSC selection process, the purity of iPSCs was enhanced, while simultaneously eliminating the need for single-cell subcloning. These iPSCs generated via the dual processing robotic approach demonstrated a purity 3.7 times greater than that of the conventional manual methods. In addition, the automatically produced human iPSCs exhibited typical pluripotent transcriptional profiles, differentiation potential, and karyotypes. In conclusion, this robotic method could offer a promising solution for the automated isolation or purification of lineage-specific cells derived from iPSCs, thereby accelerating the development of personalized medicines.
WOS关键词PLURIPOTENT STEM-CELL ; E-CADHERIN ; GENERATION ; MOUSE ; DIFFERENTIATION ; EXPRESSION ; SURVIVAL ; IPSCS ; OCT4
资助项目Key Project for Instrument Development Program-Ministry of Finance[ZDYZ2012-3] ; Key Project for Instrument Development Program-Ministry of Finance[1187000169] ; Project of Instrument Development-Chinese Academy of Sciences[1187000169] ; Project of Automated Stem Cell Induction and Culture Instrument Development-Chinese Academy of Sciences[1187000170] ; Scientific Instrument Development Program-Chinese Academy of Sciences[ZDK-YYQ20210006] ; Scientific Instrument Development Program-Chinese Academy of Sciences[YJKYYQ20210042] ; Key Research Program of Chinese Academy of Sciences[O2222001] ; Guangdong Basic and Applied Basic Research Foundation[2022A1515110435] ; Guangzhou Basic and Applied Basic Research Project[2023A04J0107] ; Project funded by China Postdoctoral Science Foundation[2021M693192] ; Guangdong International Scientific Research Cooperation Project[2022A0505050037] ; Science and Technology Planning Project of Guangdong Province[2023B1212060050]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001229653300001
资助机构Key Project for Instrument Development Program-Ministry of Finance ; Project of Instrument Development-Chinese Academy of Sciences ; Project of Automated Stem Cell Induction and Culture Instrument Development-Chinese Academy of Sciences ; Scientific Instrument Development Program-Chinese Academy of Sciences ; Key Research Program of Chinese Academy of Sciences ; Guangdong Basic and Applied Basic Research Foundation ; Guangzhou Basic and Applied Basic Research Project ; Project funded by China Postdoctoral Science Foundation ; Guangdong International Scientific Research Cooperation Project ; Science and Technology Planning Project of Guangdong Province
源URL[http://dspace.imech.ac.cn/handle/311007/95414]  
专题力学研究所_国家微重力实验室
通讯作者Zhang, Xiao
作者单位1.Univ Elect Sci & Technol China, Chengdu 611731, Peoples R China
2.South China Univ Technol, Sch Light Ind & Engn, Guangzhou 510641, Peoples R China
3.Univ Southampton, Biol Sci, Univ Rd, Southampton SO17 1BJ, England
4.Guangzhou Med Univ, Guangzhou 511436, Peoples R China
5.Guangzhou Med Univ, CAS Key Lab Regenerat Biol, Guangzhou Inst Biomed & Hlth, Joint Sch Life Sci,Chinese Acad Sci, Guangzhou 511436, Peoples R China
6.Chinese Acad Sci, Inst Elect Engn, Beijing 100190, Peoples R China
7.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China
8.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xueping,Fan, Ke,Lu, Jun,et al. Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow[J]. RESEARCH,2024,7:16.
APA Chen, Xueping.,Fan, Ke.,Lu, Jun.,Zhang, Sheng.,Dong, Jianhua.,...&龙勉.(2024).Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow.RESEARCH,7,16.
MLA Chen, Xueping,et al."Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow".RESEARCH 7(2024):16.

入库方式： OAI收割

来源：力学研究所

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