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DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process

文献类型：期刊论文

作者Wang, Xinxing8,10,11,12; Wei, Yuheng9; Cong, Rihao3,5,6,8,11,12; Wang, Wei5,7,11,12; Qi, Haigang5,6,8,11,12; Zhang, Guofan4,6,8,11,12; Pan, Ying1,9; Li, Li2,4,5,6,7,11,12
刊名AQUACULTURE
出版日期2024-03-30
卷号583页码:9
关键词Oysters Quality losses Energy metabolism DNA methylation Reproductive process AMPK signal pathway
ISSN号0044-8486
DOI10.1016/j.aquaculture.2024.740595
通讯作者Pan, Ying(yingpan@gxu.edu.cn) ; Li, Li(lili@qdio.ac.cn)
英文摘要Excessive energy consumption during reproductive process seriously reduces the nutritive value of oysters. Significant reduction of meat quality was detected in condition index and glycogen content in postspawning oysters. In order to investigate the molecular mechanism underlying this variation in quality, a conjoint analysis of DNA methylation and RNA sequencing was conducted in this study. Oysters exhibited divergent gene expression profiles during reproduction, with a particular acceleration observed in glycogen and fatty acid metabolism related pathways in postspawning oysters, including glycolysis/gluconeogenesis, TCA cycle and fatty acid degradation. Changes in genomic DNA methylation between prespawning and postspawning oysters were also detected, with oysters after spawning exhibited increased methylation trend in promoter, genebody and downstream regions. Significantly, differently methylated genes were enriched in AMPK signal pathway, and differently methylated sites of AMPK alpha were identified. Combined with the gene expression variation in energy metabolism network dominated by AMPK, it can be inferred that DNA methylation could regulate energy metabolism homeostasis through influencing the gene expression of AMPK signal pathway. These findings suggested that DNA methylation-mediated energy metabolism plays a crucial role in regulating oyster glycogen content and meat quality during reproduction. This study revealed the epigenetic mechanism of quality variation during oyster reproduction for the first time.
WOS关键词CRASSOSTREA-GIGAS ; CONDITION INDEX ; IDENTIFICATION ; EPIGENETICS ; PATHWAY
资助项目National Natural Science Foundation of China[42306108] ; National Key Research and Development Program of China[2022YFD2400301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050402] ; Key Research and Development Program of Shandong[2022LZGC015] ; China Agriculture Research System of MOF and MARA[CARS -49] ; Key Technology Research and Industrialization Demonstration Projects of Qingdao[22-3-3-hygg-2-hy]
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:001172925300001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/184786]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Pan, Ying; Li, Li
作者单位1.Guangxi Univ, Coll Anim Sci & Technol, Nanning, Guangxi, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China
5.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao 266000, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
8.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
9.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China
10.Shandong Univ, Sch Life Sci, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Xinxing,Wei, Yuheng,Cong, Rihao,et al. DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process[J]. AQUACULTURE,2024,583:9.
APA Wang, Xinxing.,Wei, Yuheng.,Cong, Rihao.,Wang, Wei.,Qi, Haigang.,...&Li, Li.(2024).DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process.AQUACULTURE,583,9.
MLA Wang, Xinxing,et al."DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process".AQUACULTURE 583(2024):9.

入库方式： OAI收割

来源：海洋研究所

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