Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Hu, Jie5,6,7; Li, Shihao3,4,6,7; Miao, Miao2,6,7 ; Li, Fuhua1,3,4,6,7
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2024-05-01 |
| 卷号 | 154页码:9 |
| 关键词 | Crustin Antibacterial activity Agglutination Phagocytic ability Opsonin |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2024.105146 |
| 通讯作者 | Li, Shihao(lishihao@qdio.ac.cn) |
| 英文摘要 | Microbial drug resistance is becoming increasingly severe due to antibiotic abuse. The development and utilization of antimicrobial peptides is one of the important ways to solve this difficult problem. Crustins are a family of antimicrobial peptides that play important roles in the innate immune system of crustaceans. Several types of crustins exist in shrimp and their activities vary greatly. In the present study, we studied the immune function of one newly identified crustin and found that the type VI crustin encoding gene in Litopenaeus vannamei (LvCrustinVI) was mainly expressed in gills. Its expression was significantly up-regulated after Vibrio parahaemolyticus infection and knockdown of the gene promoted Vibrio proliferation in the hepatopancreas of shrimp, indicating that LvCrustinVI was involved in pathogens infection. The recombinant LvCrustinVI (rLvCrustinVI) showed strong inhibitory activities against both Gram-negative and Gram-positive bacteria, and exhibited binding activities with the bacteria and bacterial polysaccharides including Glu, LPS and PGN. In the presence of Ca2+, rLvCrustinVI showed a strong agglutination effect on V. parahaemolyticus and could significantly enhance the phagocytic ability of shrimp hemocytes against V. parahaemolyticus. In conclusion, LvCrustinVI played important roles as antimicrobial peptide and opsonin in the innate immune defense of L. vannamei. The study enriched our understanding of the functional activity of Crustin and provides an important basis for the development and utilization of antimicrobial peptides. |
| WOS关键词 | DOMAIN-CONTAINING PROTEIN ; MUD CRAB ; CRUSTIN ; IMMUNITY ; BINDING |
| 资助项目 | Key Program of National Natural Science Foundation of China[31830100] ; General Program of National Natural Science Foundation of China[31972829] ; Earmarked fund for CARS-48 ; Taishan Scholars Program |
| WOS研究方向 | Fisheries ; Immunology ; Veterinary Sciences ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:001186776400001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184795]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Shihao |
| 作者单位 | 1.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 5.Ocean Univ China, Key Lab Marine Environm & Ecol, Minist Educ, Qingdao 266100, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Jie,Li, Shihao,Miao, Miao,et al. Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2024,154:9. |
| APA | Hu, Jie,Li, Shihao,Miao, Miao,&Li, Fuhua.(2024).Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,154,9. |
| MLA | Hu, Jie,et al."Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 154(2024):9. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。