Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium
文献类型：期刊论文
| 作者 | Zheng, Rikuan2,3,4,5 ; Wang, Chong2,3,4,5; Sun, Chaomin1,2,3,4,5
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| 刊名 | MBIO
 |
| 出版日期 | 2024-02-28 |
| 页码 | 16 |
| 关键词 | deep-sea Chloroflexota sulfur assimilation proteomics in situ transcriptomics metagenomics metatranscriptomics |
| ISSN号 | 2150-7511 |
| DOI | 10.1128/mbio.00004-24 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| 英文摘要 | Chloroflexota bacteria are abundant and globally distributed in various deep-sea ecosystems. It has been reported based on metagenomics data that two deep-sea Chloroflexota lineages (the SAR202 group and Dehalococcoidia class) have the potential to drive sulfur cycling. However, the absence of cultured Chloroflexota representatives is a significant bottleneck toward understanding their contribution to the deep-sea sulfur cycling. In this study, we find that Phototrophicus methaneseepsis ZRK33 isolated from deep-sea sediment has a heterotrophic lifestyle and can assimilate sulfate and thiosulfate. Using combined physiological, genomic, proteomic, and in situ transcriptomic methods, we find that strain ZRK33 can perform assimilatory sulfate reduction in both laboratory and deep-sea conditions. Metabolism of sulfate or thiosulfate by strain ZRK33 significantly promotes the transport and degradation of various macromolecules and thereby stimulates the energy production. In addition, metagenomic results show that genes associated with assimilatory and dissimilatory sulfate reduction are ubiquitously distributed in the metagenome-assembled genomes of Chloroflexota members derived from deep-sea sediments. Metatranscriptomic results also show that the expression levels of related genes are upregulated, strongly suggesting that Chloroflexota bacteria may play undocumented roles in deep-sea sulfur cycling. IMPORTANCE The cycling of sulfur is one of Earth's major biogeochemical processes and is closely related to the energy metabolism of microorganisms living in the deep-sea cold seep and hydrothermal vents. To date, some of the members of Chloroflexota are proposed to play a previously unrecognized role in sulfur cycling. However, the sulfur metabolic characteristics of deep-sea Chloroflexota bacteria have never been reported, and remain to be verified in cultured deep-sea representatives. Here, we show that the deep-sea Chloroflexota bacterium ZRK33 can perform sulfate assimilation in both laboratory and deep-sea conditions, which expands our knowledge of the sulfur metabolic potential of deep-sea Chloroflexota bacteria. We also show that the genes associated with assimilatory and dissimilatory sulfate reduction ubiquitously distribute in the deep-sea Chloroflexota members, providing hints to the roles of Chloroflexota bacteria in deep-sea sulfur biogeochemical cycling. |
| WOS关键词 | MICROBIAL ABUNDANCE ; SULFATE REDUCTION ; MARINE-SEDIMENTS ; COMPLEX ; PROTEIN ; GENOMICS ; TIGRFAMS ; QMOABC ; LIGHT |
| 资助项目 | Science and Technology Innovation Project of Laoshan Laboratory ; NSFC Innovative Group Grant[42221005] ; Major Research Plan of the National Natural Science Foundation[92351301] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Shandong Provincial Natural Science Foundation[ZR2023QD010] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Key Collaborative Research Program of the Alliance of International Science Organizations ; Key deployment projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Taishan Scholars Program[tsqn202312264] ; [LSKJ202203103] ; [2022QNLM030004-3] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001177605500001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184819]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Rikuan,Wang, Chong,Sun, Chaomin. Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium[J]. MBIO,2024:16. |
| APA | Zheng, Rikuan,Wang, Chong,&Sun, Chaomin.(2024).Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium.MBIO,16. |
| MLA | Zheng, Rikuan,et al."Deep-sea in situ and laboratory multi-omics provide insights into the sulfur assimilation of a deep-sea Chloroflexota bacterium".MBIO (2024):16. |
入库方式： OAI收割
来源：海洋研究所
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