Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner
文献类型：期刊论文
| 作者 | Wang, Yujian3,4,5 ; Luo, Jingchang2,3,4,5; Guan, Xiaolu2,3,4,5; Zhao, Yan1; Sun, Li2,3,4,5
|
| 刊名 | CELL DEATH DISCOVERY
 |
| 出版日期 | 2024-03-08 |
| 卷号 | 10期号:1页码:9 |
| DOI | 10.1038/s41420-024-01887-7 |
| 通讯作者 | Zhao, Yan(zhaoyan1316@163.com) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Bacillus cereus is a clinically significant foodborne pathogen that causes severe gastrointestinal and non-gastrointestinal disease. Cereolysin O (CLO) is a putative virulence factor of B. cereus, and its function remains to be investigated. In this study, we examined the biological activity of CLO from a deep sea B. cereus isolate. CLO was highly toxic to mammalian cells and triggered pyroptosis through NLRP3 inflammasome-mediated caspase 1 and gasdermin D activation. CLO-induced cell death involved ROS accumulation and K+ efflux, and was blocked by serum lipids. CLO bound specifically to cholesterol, and this binding was essential to CLO cytotoxicity. The structural integrity of the three tryptophan residues in the C-terminal undecapeptide was vital for CLO to interact with membrane lipids and cause membrane perforation. Taken together, these results provided new insights into the molecular mechanism of B. cereus CLO-mediated cytotoxicity. |
| WOS关键词 | TOXIN PERFRINGOLYSIN-O ; GASDERMIN D ; INFLAMMATORY CASPASES ; NLRP3 INFLAMMASOME ; NALP3 INFLAMMASOME ; OXIDATIVE STRESS ; PATHOGENICITY ; ACTIVATION ; CYTOLYSINS ; GSDMD |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001181435500001 |
| 出版者 | SPRINGERNATURE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184822]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhao, Yan; Sun, Li |
| 作者单位 | 1.Tsinghua Univ, Sch Med, Tsinghua Peking Joint Ctr Life Sci, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Inst Oceanol, Chinese Acad Sci, CAS Ctr Ocean Mega Sci, CAS, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yujian,Luo, Jingchang,Guan, Xiaolu,et al. Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner[J]. CELL DEATH DISCOVERY,2024,10(1):9. |
| APA | Wang, Yujian,Luo, Jingchang,Guan, Xiaolu,Zhao, Yan,&Sun, Li.(2024).Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner.CELL DEATH DISCOVERY,10(1),9. |
| MLA | Wang, Yujian,et al."Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner".CELL DEATH DISCOVERY 10.1(2024):9. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。