Comprehensive Multi-omics Approaches Provide Insights to Summer Mortality in the Clam Meretrix petechialis
文献类型：期刊论文
| 作者 | Tian, Jing1,4,5 ; Wang, Hongxia3,4,5; Huan, Pin2,3,4,5; Yue, Xin3,4,5; Liu, Baozhong1,2,3,4,5
|
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2024-03-14 |
| 页码 | 15 |
| 关键词 | Summer mortality Multi-omics analysis Vibrio parahaemolyticus High temperature Meretrix petechialis |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-024-10304-0 |
| 通讯作者 | Liu, Baozhong(bzliu@qdio.ac.cn) |
| 英文摘要 | Bivalve mass mortalities have been reported worldwide, which not only can be explained as a result of pathogen infection, but may reflect changes in environments. Although these episodes were often reported, there was limited information concerning the molecular responses to various stressors leading to summer mortality. In the present work, RNA sequencing (RNA-seq), tandem mass tagging (TMT)-based quantitative proteomics, and 16S rRNA sequencing were used to explore the natural outbreak of summer mortality in the clam Meretrix petechialis. We identified a total of 172 differentially expressed genes (DEGs) and 222 differentially expressed proteins (DEPs) in the diseased group compared to the normal group. The inconsistent expression profiles of immune DEGs/DEPs may be due to the immune dysregulation of the diseased clams. Notably, 11 solute carrier family genes were found among the top 20 down-regulated genes in the diseased group, indicating that weakened transmembrane transport ability might occur in the diseased clams. Integration analysis of transcriptomic and proteomic results showed that many metabolic processes such as "arginine and proline metabolism" and "tyrosine metabolism" were inhibited in the diseased group, suggesting metabolic inhibition. Moreover, 16S rRNA sequencing revealed that the microbial composition of clam hepatopancreas was disordered in the diseased group. The comparison of DEGs expression between the natural summer mortality event and an artificial challenge experiment involving both Vibrio infection and heat stress revealed 9/15 genes showing similar expression trends between the two conditions, suggesting that the summer mortality might be caused by a combination of high temperature and Vibrio infection. These results would deepen our understanding of summer mortality and provide candidate resistance markers for clam resistance breeding. |
| WOS关键词 | CRASSOSTREA-GIGAS ; PACIFIC OYSTERS ; VIBRIO RESISTANCE ; MASS MORTALITIES ; CLIMATE-CHANGE ; GENE ; IDENTIFICATION ; POLYMORPHISMS ; TEMPERATURE ; INFECTIONS |
| 资助项目 | Chinese Academy of Sciences |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001183465300002 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184831]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Baozhong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Jing,Wang, Hongxia,Huan, Pin,et al. Comprehensive Multi-omics Approaches Provide Insights to Summer Mortality in the Clam Meretrix petechialis[J]. MARINE BIOTECHNOLOGY,2024:15. |
| APA | Tian, Jing,Wang, Hongxia,Huan, Pin,Yue, Xin,&Liu, Baozhong.(2024).Comprehensive Multi-omics Approaches Provide Insights to Summer Mortality in the Clam Meretrix petechialis.MARINE BIOTECHNOLOGY,15. |
| MLA | Tian, Jing,et al."Comprehensive Multi-omics Approaches Provide Insights to Summer Mortality in the Clam Meretrix petechialis".MARINE BIOTECHNOLOGY (2024):15. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。