Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens
文献类型：期刊论文
| 作者 | Wei, Xiaotong4; Hu, Yuanyuan4; Sun, Chaomin1,2,3; Wu, Shimei4 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 出版日期 | 2024-03-01 |
| 卷号 | 72期号:10页码:5283-5292 |
| 关键词 | antimicrobial peptides foodborne pathogen Bacillus transcriptomic analysis antimicrobialmechanism |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.4c00573 |
| 通讯作者 | Wu, Shimei(shimeiwu2016@126.com) |
| 英文摘要 | The increasing emergence of multidrug-resistant pathogens and development of biopreservatives in food industries has increased the demand of novel and safe antimicrobial agents. In this study, a marine bacterial strain Bacillus licheniformis M1 was isolated and exhibited obvious antimicrobial activities against foodborne pathogens, especially against methicillin-resistant Staphylococcus aureus. The antimicrobial agent was purified and identified as a novel antimicrobial peptide, which was designated as bacipeptin, and the corresponding mechanism was further investigated by electron microscopy observation and transcriptomic analysis with biochemical validation. The results showed that bacipeptin could reduce the virulence of methicillin-resistant Staphylococcus aureus and exerted its antimicrobial activity by interfering with histidine metabolism, inducing the accumulation of reactive oxygen species and down-regulating genes related to Na+/H+ antiporter and the cell wall, thus causing damage to the cell wall and membrane. Overall, our study provides a novel natural product against foodborne pathogens and discloses the corresponding action mechanism. |
| WOS关键词 | NA+/H+ ANTIPORTER ; STAPHYLOCOCCUS-AUREUS ; HISTIDINE ; FOOD ; PREVALENCE |
| 资助项目 | Science and Technology Innovation Project of Laosh an Laboratory[LSKJ202203103] ; Science and Technology Innovation Project of Laosh an Laboratory[2022QNLM030004-3] ; NSFC Innovative Group Grant[42221005] ; Shandong Provincial Natural Science Foundation[ZR2021MD099] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Major Research Plan of the National Natural Science Foundation[92351301] ; Taishan Scholars Program[tstp20230637] |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001179315000001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184866]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wu, Shimei |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 4.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Xiaotong,Hu, Yuanyuan,Sun, Chaomin,et al. Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(10):5283-5292. |
| APA | Wei, Xiaotong,Hu, Yuanyuan,Sun, Chaomin,&Wu, Shimei.(2024).Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(10),5283-5292. |
| MLA | Wei, Xiaotong,et al."Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.10(2024):5283-5292. |
入库方式： OAI收割
来源：海洋研究所
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