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Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress

文献类型：期刊论文

作者Huang, Danlin1,4,5; Tian, Chao1,4,5; Sun, Zhenjie1,4,5; Niu, Jianfeng4,5; Wang, Guangce2,3,4,5
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2024-03-01
卷号78页码:11
关键词High temperatures Antioxidant enzymes hsp70 Heat shock transcription factor Pyropia yezoensis
ISSN号2211-9264
DOI10.1016/j.algal.2023.103375
通讯作者Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要High temperatures have been regarded as one of the main factors limiting Pyropia yezoensis cultivation in recent years. Both the heat shock proteins and antioxidant enzymes genes were activated timely before heat damage to the cell. Here, the activities of different antioxidant enzymes under different conditions, including HSF inhibitor, CaM inhibitor, and HSP70 protein inhibitor were determined. Then, RNA-seq was performed using samples that had been pretreated with corresponding chemicals under high temperature conditions. The principal component analysis inferred that the shift of gene transcription patterns under high temperature conditions was closely related to HSF since the addition of QR suppressed the changes in gene transcription pattern. The hsp70 genes and antioxidant enzyme genes were categorized into three groups by the method of WGCNA. And the HSE sites were identified using plantPAN and FIMO in most genes of the categories showed relationship to HSF. Quantitative RT-PCR analysis of designated genes (FMPK >10) indicated that the expression of hsp70 and antioxidant enzyme genes in Py. yezoensis showed various transcriptional patterns, including upregulation, downregulation, and no significant changes in response to high temperature stress. The classification between qRT-PCR and WGCNA results showed a certain consistency. Considered the results above together, it was speculated that there were different HSFs regulated the expression of different genes of hsp70 and antioxidant enzymes, and HSP70 might also regulate the expression of anti-oxidase genes under the conditions of long-term stress. Additionally, transcription factors other than HSF might be involved in the high temperature stress response. The results indicated the complexity of heat stress response mechanisms in Py. yezoensis.
WOS关键词HEAT-SHOCK FACTOR ; TRANSCRIPTION FACTORS ; MOLECULAR CHAPERONES ; EXPRESSION ; TOLERANCE ; HSP70 ; ARABIDOPSIS ; BINDING ; HSF1 ; PROTEIN
资助项目Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; National Natural Science Foundation of China[42376091] ; China Agriculture Research System of MOF and MARA[CARS -50]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:001174430400001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/184896]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Niu, Jianfeng; Wang, Guangce
作者单位1.Qingdao Agr Univ, Qingdao, Peoples R China
2.Laoshan Lab, Qingdao, Peoples R China
3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
5.Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Danlin,Tian, Chao,Sun, Zhenjie,et al. Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2024,78:11.
APA Huang, Danlin,Tian, Chao,Sun, Zhenjie,Niu, Jianfeng,&Wang, Guangce.(2024).Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,78,11.
MLA Huang, Danlin,et al."Potential synergistic regulation of hsp70 and antioxidant enzyme genes in Pyropia yezoensis under high temperature stress".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 78(2024):11.

入库方式： OAI收割

来源：海洋研究所

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