Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus
文献类型：期刊论文
| 作者 | Li, Rongmei2,3,4,5,6; Su, Zhenjie1,2,3,4,5; Sun, Chaomin1,2,3,4,5; Wu, Shimei6 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2024-04-17 |
| 卷号 | 90期号:4页码:25 |
| 关键词 | antibacteria biosynthesis alternariol and derivative marine fungus multidrug-resistant bacteria |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.00058-24 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) ; Wu, Shimei(shimeiwu2016@126.com) |
| 英文摘要 | Alternaria alternata FB1 is a marine fungus identified as a candidate for plastic degradation in our previous study. This fungus has been recently shown to produce secondary metabolites with significant antimicrobial activity against various pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and the notorious aquaculture pathogen Vibrio anguillarum. The antibacterial compounds were purified and identified as alternariol (AOH) and its derivative, alternariol monomethyl ether (AME). We found that AOH and AME primarily inhibited pathogenic bacteria (MRSA or V. anguillarum) by disordering cell division and some other key physiological and biochemical processes. We further demonstrated that AOH could effectively inhibit the unwinding activity of MRSA topoisomerases, which are closely related to cell division and are the potential action target of AOH. The antibacterial activities of AOH and AME were verified by using zebrafish as the in vivo model. Notably, AOH and AME did not significantly affect the viability of normal human liver cells at concentrations that effectively inhibited MRSA or V. anguillarum. Finally, we developed the genetic operation system of A. alternata FB1 and blocked the biosynthesis of AME by knocking out omtI (encoding an O-methyl transferase), which facilitated A. alternata FB1 to only produce AOH. The development of this system in the marine fungus will accelerate the discovery of novel natural products and further bioactivity study. IMPORTANCE More and more scientific reports indicate that alternariol (AOH) and its derivative alternariol monomethyl ether (AME) exhibit antibacterial activities. However, limited exploration of their detailed antibacterial mechanisms has been performed. In the present study, the antibacterial mechanisms of AOH and AME produced by the marine fungus Alternaria alternata FB1 were disclosed in vitro and in vivo. Given their low toxicity on the normal human liver cell line under the concentrations exhibiting significant antibacterial activity against different pathogens, AOH and AME are proposed to be good candidates for developing promising antibiotics against methicillin-resistant Staphylococcus aureus and Vibrio anguillarum. We also succeeded in blocking the biosynthesis of AME, which facilitated us to easily obtain pure AOH. Moreover, based on our previous results, A. alternata FB1 was shown to enable polyethylene degradation. |
| WOS关键词 | ENDOPHYTIC FUNGUS ; INHIBITORS ; ALKALOIDS ; STRESS |
| 资助项目 | Science and Technology Innovation Project of Laoshan Laboratory ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; NSFC Innovative Group Grant[42221005] ; National Natural Science Foundation of China[92351301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Key Collaborative Research Program of the Alliance of International Science Organizations ; Key deployment projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Taishan Scholars Program[tstp20230637] ; [2022QNLM030004-3] ; [LSKJ202203103] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001183409600001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/185067]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Sun, Chaomin; Wu, Shimei |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 6.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Rongmei,Su, Zhenjie,Sun, Chaomin,et al. Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2024,90(4):25. |
| APA | Li, Rongmei,Su, Zhenjie,Sun, Chaomin,&Wu, Shimei.(2024).Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,90(4),25. |
| MLA | Li, Rongmei,et al."Antibacterial insights into alternariol and its derivative alternariol monomethyl ether produced by a marine fungus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 90.4(2024):25. |
入库方式： OAI收割
来源：海洋研究所
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