Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco
文献类型：期刊论文
| 作者 | Yang, Luhua9,10; Guo, Yuan8; Yang, Hui6,7,8 ; Li, Shun5,9; Zhang, Yunzeng4; Gao, Cheng3; Wei, Tian2; Hao, Likai1,6,8
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2024-03-01 |
| 卷号 | 15页码:13 |
| 关键词 | plant microbiota tobacco microbiome disease resistance positive selection biocontrol |
| DOI | 10.3389/fmicb.2024.1361883 |
| 通讯作者 | Hao, Likai(haolikai@mail.gyig.ac.cn) |
| 英文摘要 | The plant microbiota is believed to be an accessory genome that extends plant functions, forming holobionts together with the host plant. Plant disease resistance, therefore, is inextricably linked with plant microbiota, which play important roles in plant growth and health. To explore the relationship between plant microbiota and disease resistance, we investigated the tobacco microbiome of two varieties with contrasting disease-resistance levels to bacterial wilt and black shank diseases. Comparative microbiome analysis indicated that the resistant variety assembled a distinct microbiota with higher network complexity and diversity. While Pseudomonas and Ensifer, which contain biocontrol and beneficial members, were enriched in the rhizosphere of the resistant variety, Ralstonia, a genus including the known causative pathogen, was enriched in the susceptible variety. Metagenome sequencing revealed that biocontrol functions, such as hydrogen cyanide synthase, pyochelin biosynthesis, and arthrofactin-type cyclic lipopeptide synthetase, were more abundant in the resistant variety. Further analysis indicated that contigs encoding the corresponding genes were mostly assigned to Pseudomonas. Among all the metagenome-assembled genomes, positive selection was suggested in the genome assigned to Pseudomonas only in the rhizosphere of the resistant variety. The search of biosynthetic gene clusters in the Pseudomonas genome revealed a non-ribosomal peptide synthetase, the compound of which was brabantamide A, with known antimicrobial activity. Collectively, our study suggests that the plant microbiota might be involved in microbe-mediated disease resistance. Particularly, our results highlight Pseudomonas in the rhizosphere of the disease-resistant variety as a promising biocontrol candidate. Our study may facilitate further screening of bacterial isolates and the targeted design of microbial communities. |
| WOS关键词 | PATHOGEN ; PSEUDOMONAS ; DIVERSITY ; BACTERIAL ; INHIBITORS ; SB-253514 ; STRAIN ; GROWTH ; SOIL |
| 资助项目 | Guizhou Provincial Department of Science and Technology |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001185098200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/184816]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Hao, Likai |
| 作者单位 | 1.CAS Ctr Excellence Quaternary Sci & Global Change, Xian, Peoples R China 2.Bei Bu Zhan Qu CDC, Shenyang, Peoples R China 3.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing, Peoples R China 4.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou, Peoples R China 5.CAS Haixi Ind Technol Innovat Ctr Beilun, Zhejiang Key Lab Urban Environm Proc & Pollut Cont, Ningbo 315800, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China 7.Guizhou Acad Tobacco Sci, Guiyang, Peoples R China 8.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang, Peoples R China 9.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Ningbo Urban Environm Observat & Res Stn, Xiamen, Peoples R China 10.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Luhua,Guo, Yuan,Yang, Hui,et al. Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco[J]. FRONTIERS IN MICROBIOLOGY,2024,15:13. |
| APA | Yang, Luhua.,Guo, Yuan.,Yang, Hui.,Li, Shun.,Zhang, Yunzeng.,...&Hao, Likai.(2024).Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco.FRONTIERS IN MICROBIOLOGY,15,13. |
| MLA | Yang, Luhua,et al."Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco".FRONTIERS IN MICROBIOLOGY 15(2024):13. |
入库方式： OAI收割
来源：海洋研究所
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