Simultaneous two-photon imaging and wireless EEG recording in mice
文献类型：期刊论文
| 作者 | Kim, Bowon5,6; Ding, Weihua6; Yang, Liuyue6; Chen, Qian2,3,4; Mao, Jianren6; Feng, Guoping3,4; Choi, Jee Hyun5; Shen, Shiqian1,6 |
| 刊名 | HELIYON
 |
| 出版日期 | 2024-03-15 |
| 卷号 | 10期号:5页码:9 |
| 关键词 | Two-photon Electroencephalogram (EEG) Anesthesia Isoflurane Global brain state Multi-dimensional brain activity |
| DOI | 10.1016/j.heliyon.2024.e25910 |
| 通讯作者 | Shen, Shiqian(sshen2@mgh.harvard.edu) |
| 英文摘要 | Background: In vivo two-photon imaging is a reliable method with high spatial resolution that allows observation of individual neuron and dendritic activity longitudinally. Neurons in local brain regions can be influenced by global brain states such as levels of arousal and attention that change over relatively short time scales, such as minutes. As such, the scientific rigor of investigating regional neuronal activities could be enhanced by considering the global brain state. New method: In order to assess the global brain state during in vivo two-photon imaging, CBRAIN (collective brain research platform aided by illuminating neural activity), a wireless EEG collecting and labeling device, was controlled by the same computer of two-photon microscope. In an experiment to explore neuronal responses to isoflurane anesthesia through two-photon imaging, we investigated whether the response of individual cells correlated with concurrent EEG changes induced by anesthesia. Results: In two-photon imaging, calcium activities of the excitatory neurons in the primary somatosensory cortex disappeared in about 30s after to the initiation of isoflurane anesthesia. The simultaneously recorded EEG showed various transitional activity for about 7 min from the initiation of anesthesia and continued with burst and suppression alternating pattern thereafter. As such, there was a dissociation between excitatory neuron activity of the primary somatosensory cortex and the global brain activity under anesthesia. Comparison with existing method(s): Existing methods to combine two-photon and EEG recording used wired EEG recording. In this study, wireless EEG was used in conjunction with two-photon imaging, facilitated by CBRAIN. More importantly, built-in algorithms of the CBRAIN can automatically detect brain state such as sleep. The codes used for EEG classification are easy to use, with no prior experience required. Conclusion: Simultaneous recording of wireless EEG and two-photon imaging provides a practical way to capture individual neuronal activities with respect to global brain state in an experimental set-up. |
| WOS关键词 | FLUORESCENT SENSORS ; SLEEP ; OSCILLATIONS ; STATE ; AWAKE |
| 资助项目 | NIH[R35GM128692] ; NIH[R01 AG 070141] ; NIH[R61 NS126029] ; NIH[HI19C075300] ; NSF EAGER Award ; Borsook Project ; Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea[2334666] ; [R03 AG067947] ; [R61NS116423] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001209696400001 |
| 出版者 | CELL PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/310968]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Shen, Shiqian |
| 作者单位 | 1.149 13th St,Room 6224, Boston, MA 02129 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Shanghai, Peoples R China 3.MIT, Dept Brain & Cognit Sci, Cambridge, MA USA 4.MIT, McGovern Inst Brain Res, Cambridge, MA USA 5.Korea Inst Sci & Technol, Ctr Neurosci, Seoul, South Korea 6.Harvard Med Sch, Dept Anesthesia Crit Care & Pain Med, Ctr Translat Pain Res, Massachusetts Gen Hosp, Boston, MA USA |
| 推荐引用方式 GB/T 7714 | Kim, Bowon,Ding, Weihua,Yang, Liuyue,et al. Simultaneous two-photon imaging and wireless EEG recording in mice[J]. HELIYON,2024,10(5):9. |
| APA | Kim, Bowon.,Ding, Weihua.,Yang, Liuyue.,Chen, Qian.,Mao, Jianren.,...&Shen, Shiqian.(2024).Simultaneous two-photon imaging and wireless EEG recording in mice.HELIYON,10(5),9. |
| MLA | Kim, Bowon,et al."Simultaneous two-photon imaging and wireless EEG recording in mice".HELIYON 10.5(2024):9. |
入库方式： OAI收割
来源：上海药物研究所
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