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Euphohelioscopin A enhances NK cell antitumor immunity through GSDME-triggered pyroptosis

文献类型：期刊论文

作者Gong, Chenyuan4,5; Mu, Hongyan2,3; Luo, Jiaojiao4,5; Zhang, Rujun3; Hu, Dan1; Chen, Zhenhua3; Fang, Cheng4,5; Chen, Zhongxian2,3; Zhu, Xinxue4,5; Yao, Chao4,5
刊名JOURNAL OF LEUKOCYTE BIOLOGY
出版日期2024-03-08
页码11
关键词antitumor immunity euphohelioscopin A GSDME NK cells pyroptosis
ISSN号0741-5400
DOI10.1093/jleuko/qiae055
通讯作者Zhao, Weimin(wmzhao@simm.ac.cn) ; Zhu, Shiguo(zhushiguo@shutcm.edu.cn)
英文摘要Immune evasion by cancer cells poses a significant challenge for natural killer cell-based immunotherapy. Pyroptosis, a newly discovered form of programmed cell death, has shown great potential for enhancing the antitumor immunity of natural killer cells. Consequently, targeting pyroptosis has become an attractive strategy for boosting natural killer cell activity against cancer. In this study, various assays were conducted, including natural killer cell cytotoxicity assays, flow cytometry, xenograft tumor models, real-time polymerase chain reaction, and enzyme-linked immunosorbent assay, to assess natural killer cell-mediated cell killing, as well as gene and protein expressions. The results indicated that euphohelioscopin A, a potential pyroptosis activator, enhances natural killer cell-mediated lysis of tumor cells, resulting in inhibiting tumor growth that could be reversed by natural killer cell depletion. Furthermore, we found that euphohelioscopin A significantly enhanced IFN gamma production in natural killer cells and synergistically upregulated GSDME with IFN gamma in cancer cells. Euphohelioscopin A also increased the cleavage of GSDME, promoting granzyme B-induced pyroptosis, which could be reversed by GSDME knockdown and IFN gamma blockade. Overall, the findings suggested that euphohelioscopin A enhanced natural killer cell-mediated killing of cancer cells by triggering pyroptosis, making euphohelioscopin A a promising pyroptosis activator with great potential for use in natural killer cell-based cancer immunotherapy. Euphohelioscopin A promotes natural killer cell-mediated tumor killing by triggering GSDME-induced pyroptosis.
WOS关键词ACTIVATOR ; CANCER ; GAMMA
资助项目National Natural Science Foundation of China[81903932] ; National Natural Science Foundation of China[81803933] ; National Natural Science Foundation of China[81903848] ; National Natural Science Foundation of China[82373908] ; National Natural Science Foundation of China[22377137] ; Natural Science Foundation of Shanghai[19ZR1457500]
WOS研究方向Cell Biology ; Hematology ; Immunology
语种英语
WOS记录号WOS:001209644800001
出版者OXFORD UNIV PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/310983]  
专题中国科学院上海药物研究所
通讯作者Zhao, Weimin; Zhu, Shiguo
作者单位1.Shanghai Univ Tradit Chinese Med, Sch Acupuncture Moxibust & Tuina, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, 1 Yanqihu East Rd, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Res Ctr, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
4.Shanghai Univ Tradit Chinese Med, Sch Integrat Med, Dept Immunol & Pathogen Biol, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China
5.Shanghai Univ Tradit Chinese Med, Ctr Tradit Chinese Med & Immunol Res, Sch Integrat Med, 1200 Cai Lun Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Gong, Chenyuan,Mu, Hongyan,Luo, Jiaojiao,et al. Euphohelioscopin A enhances NK cell antitumor immunity through GSDME-triggered pyroptosis[J]. JOURNAL OF LEUKOCYTE BIOLOGY,2024:11.
APA Gong, Chenyuan.,Mu, Hongyan.,Luo, Jiaojiao.,Zhang, Rujun.,Hu, Dan.,...&Zhu, Shiguo.(2024).Euphohelioscopin A enhances NK cell antitumor immunity through GSDME-triggered pyroptosis.JOURNAL OF LEUKOCYTE BIOLOGY,11.
MLA Gong, Chenyuan,et al."Euphohelioscopin A enhances NK cell antitumor immunity through GSDME-triggered pyroptosis".JOURNAL OF LEUKOCYTE BIOLOGY (2024):11.

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来源：上海药物研究所

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