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Exploring the mechanism of contact-dependent cell-cell communication on chemosensitivity based on single-cell high-throughput drug screening platform

文献类型：期刊论文

作者Jiang, Yue6,7; Ren, Xuelian6; Liu, Guobin6; Chen, Shulei7; Hao, Ming7; Deng, Xinran7; Huang, He1,5,6; Liu, Kun2,3,4,7
刊名TALANTA
出版日期2024-06-01
卷号273页码:8
关键词Microfluidic chip High-throughput drug screening Quantitative proteomics Contact-dependent cell-cell communication Tumour single cells
ISSN号0039-9140
DOI10.1016/j.talanta.2024.125869
通讯作者Huang, He(hhuang@simm.ac.cn) ; Liu, Kun(kliu@mail.neu.edu.cn)
英文摘要High-throughput drug screening (HTDS) has significantly reduced the time and cost of new drug development. Nonetheless, contact-dependent cell-cell communication (CDCCC) may impact the chemosensitivity of tumour cells. There is a pressing need for low-cost single-cell HTDS platforms, alongside a deep comprehension of the mechanisms by which CDCCC affects drug efficacy, to fully unveil the efficacy of anticancer drugs. In this study, we develop a microfluidic chip for single-cell HTDS and evaluate the molecular mechanisms impacted by CDCCC using quantitative mass spectrometry-based proteomics. The chip achieves high-quality drug mixing and singlecell capture, with single-cell drug screening results on the chip showing consistency with those on the 96 -well plates under varying concentration gradients. Through quantitative proteomic analysis, we deduce that the absence of CDCCC in single tumour cells can enhance their chemoresistance potential, but simultaneously subject them to stronger proliferation inhibition. Additionally, pathway enrichment analysis suggests that CDCCC could impact several signalling pathways in tumour single cells that regulate vital biological processes such as tumour proliferation, adhesion, and invasion. These results offer valuable insights into the potential connection between CDCCC and the chemosensitivity of tumour cells. This research paves the way for the development of single-cell HTDC platforms and holds the promise of advancing tumour personalized treatment strategies.
WOS关键词CANCER ; HETEROGENEITY ; RESISTANCE
资助项目The 111 Project[B16009] ; Natural Science Foundation of Shenyang City[22-315-6-01] ; Natural Science Foundation of Shanghai[23ZR1474600] ; National Natural Science Foundation of China[22277125] ; National Natural Science Foundation of China[92253306]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001208365400001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/310995]  
专题中国科学院上海药物研究所
通讯作者Huang, He; Liu, Kun
作者单位1.647 Songtao Rd, Shanghai 200120, Peoples R China
2.3-11 WenHua Rd, Shenyang 110004, Peoples R China
3.Northeastern Univ, Minist Educ, Key Lab Data Analyt & Optimizat Smart Ind, Shenyang, Peoples R China
4.Northeastern Univ, Natl Frontiers Sci Ctr Ind Intelligence & Syst Opt, Shenyang 110819, Peoples R China
5.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
7.Northeastern Univ, Sch Mech Engn & Automat, Shenyang 110819, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Yue,Ren, Xuelian,Liu, Guobin,et al. Exploring the mechanism of contact-dependent cell-cell communication on chemosensitivity based on single-cell high-throughput drug screening platform[J]. TALANTA,2024,273:8.
APA Jiang, Yue.,Ren, Xuelian.,Liu, Guobin.,Chen, Shulei.,Hao, Ming.,...&Liu, Kun.(2024).Exploring the mechanism of contact-dependent cell-cell communication on chemosensitivity based on single-cell high-throughput drug screening platform.TALANTA,273,8.
MLA Jiang, Yue,et al."Exploring the mechanism of contact-dependent cell-cell communication on chemosensitivity based on single-cell high-throughput drug screening platform".TALANTA 273(2024):8.

入库方式： OAI收割

来源：上海药物研究所

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