In this article, the problem of inferring unknown isoperimetric constraints is considered given optimal state and control trajectories that solve the optimal control problem with isoperimetric constraints. By exploiting Pontryagin’s principle, the recovery equations for unknown isoperimetric constraints are established. Under verifiable dimensionality condition and matrix rank condition, the proposed method is guaranteed to infer the unknown isoperimetric constraints exactly. Furthermore, the proposed method is extended to multiple trajectory setting. Finally, the effectiveness of the proposed method is illustrated by two simulation examples with various settings.