中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 力学研究所
  3. 中国科学院力学研究所
  4. 国家微重力实验室
Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors

文献类型：期刊论文

作者Li, Rongjie6; Liu, Daixin4,5; Ren, Meili3,4,5; Qin, Fupeng4,5; Qi, Guodong2; Tian, Dawei6; Zhang, Mingkun1,4,5; Xie, Wanyi4,5; Wang, Liang4,5; Lu, Shouqin1
刊名CHEMICAL ENGINEERING JOURNAL
出版日期2024-07-15
卷号492页码:8
关键词Single molecule integrin Solid-state nanopores divalent metal ions Oligopeptides Conformation
ISSN号1385-8947
DOI10.1016/j.cej.2024.152374
通讯作者Pan, Jun(panj@cqu.edu.cn) ; Liang, Liyuan(liangliyuan@cigit.ac.cn)
英文摘要Integrins are a large family of heterodimeric mammalian transmembrane receptors that connect extracellular matrix (ECM) and the cytoskeleton. Integrin-mediated adhesion governs the cellular adhesion, mobility, and cell mechanobiology. Investigations on different integrins in cellular and the conformation change regulated by divalent metal ions and oligopeptides have been documented, they mainly adopted the cellular assays or surface manipulation to achieve the information of ensemble integrins with proteins and small molecules. This work presents the nanopore approach for the characterization of unimolecular integrin via resistive pulse sensing. The orientation selection in distinct nanopore in size and pH condition discloses the tiny disparity of the conformation and the local surface charge distribution. The divalent metal ions could efficiently modulate the conformation alteration of integrin, in which Ca2+ and Mn2+ could activate integrin alpha L beta 2 to take larger physical dimension than Mg2+, and Ca2+ is superior to Mn2+ in the activation when integrin alpha L beta 2 is treated in the mixed metal ions. The oligopeptide cRGD (cyclic Arg-Gly-Asp) and its derivatives cRGDfk and cRGDyk show specific binding with integrin alpha V beta 3 and the complex of alpha V beta 3-cRGD exhibits largest geometry upon Ca2+-mediation with characteristic nanopore translocation feature. This work provides a platform for unimolecular integrin sensing and the modulation of the local conformation transition regulated by divalent metal ions and oligopeptides, which will be valuable for the representation of integrin-related cellular behavior and bio-function.
WOS关键词ACTIVATION
资助项目Excellent Youth Foundation of Chongqing Scientific Committee[cstc2021jcyj-jqX0030] ; West Light Foundation of the Chinese Academy of Sciences ; National Key Research and Development Program of China[2022YFB3205600] ; University of the Chinese Academy of Sciences
WOS研究方向Engineering
语种英语
WOS记录号WOS:001244390500001
资助机构Excellent Youth Foundation of Chongqing Scientific Committee ; West Light Foundation of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; University of the Chinese Academy of Sciences
源URL[http://dspace.imech.ac.cn/handle/311007/95672]  
专题力学研究所_国家微重力实验室
通讯作者Pan, Jun; Liang, Liyuan
作者单位1.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China
2.Northeast Elect Power Univ, Sch Chem Engn, Jilin 132012, Jilin, Peoples R China
3.Chongqing Jiaotong Univ, Chongqing 400014, Peoples R China
4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
6.Chongqing Univ, Coll Bioengn 10, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants,Minist, Chongqing 400044, Peoples R China
推荐引用方式
GB/T 7714		 Li, Rongjie,Liu, Daixin,Ren, Meili,et al. Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors[J]. CHEMICAL ENGINEERING JOURNAL,2024,492:8.
APA Li, Rongjie.,Liu, Daixin.,Ren, Meili.,Qin, Fupeng.,Qi, Guodong.,...&张明焜.(2024).Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors.CHEMICAL ENGINEERING JOURNAL,492,8.
MLA Li, Rongjie,et al."Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors".CHEMICAL ENGINEERING JOURNAL 492(2024):8.

入库方式： OAI收割

来源：力学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。