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Physical Contacts Between Mitochondria and WPBs Participate in WPB Maturation

文献类型：期刊论文

作者Ma, Jing3,4,5; Hao, Zhenhua1,4,5; Zhang, Yudong6,8; Li, Liuju9; Huang, Xiaoshuai10; Wang, Yu7; Chen, Liangyi9; Yang, Ge2,6,8; Li, Wei1,3,4,5
刊名ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
出版日期2024
卷号44期号:1页码:108-123
关键词endothelial cells mitochondrion organelle interaction von Willebrand factor Weibel-Palade body
ISSN号1079-5642
DOI10.1161/ATVBAHA.123.319939
通讯作者Hao, Zhenhua(nanshayu@163.com) ; Yang, Ge(ge.yang@ia.ac.cn) ; Li, Wei(liwei@bch.com.cn)
英文摘要BACKGROUND: Weibel-Palade bodies (WPBs) are endothelial cell-specific cigar-shaped secretory organelles containing various biologically active molecules. WPBs play crucial roles in thrombosis, hemostasis, angiogenesis, and inflammation. The main content of WPBs is the procoagulant protein vWF (von Willebrand factor). Physical contacts and functional cross talk between mitochondria and other organelles have been demonstrated. Whether an interorganellar connection exists between mitochondria and WPBs is unknown.METHODS: We observed physical contacts between mitochondria and WPBs in human umbilical vein endothelial cells by electron microscopy and living cell confocal microscopy. We developed an artificial intelligence-assisted method to quantify the duration and length of organelle contact sites in live cells.RESULTS: We found there existed physical contacts between mitochondria and WPBs. Disruption of mitochondrial function affected the morphology of WPBs. Furthermore, we found that Rab3b, a small GTPase on the WPBs, was enriched at the mitochondrion-WPB contact sites. Rab3b deficiency reduced interaction between the two organelles and impaired the maturation of WPBs and vWF multimer secretion.CONCLUSIONS: Our results reveal that Rab3b plays a crucial role in mediating the mitochondrion-WPB contacts, and that mitochondrion-WPB coupling is critical for the maturation of WPBs in vascular endothelial cells.
WOS关键词VON-WILLEBRAND-FACTOR ; WEIBEL-PALADE BODIES ; BIOGENESIS ; DEFICIENCY ; EXPRESSION ; SECRETION ; DELIVERY ; INSIGHTS ; FISSION
资助项目Ministry of Science and Technology of China[2019YFA0802104] ; National Natural Science Foundation of China[32293204] ; National Natural Science Foundation of China[91854110] ; National Natural Science Foundation of China[31830054] ; National Natural Science Foundation of China[91954201] ; National Natural Science Foundation of China[31971289] ; Beijing Natural Science Foundation[5192008]
WOS研究方向Hematology ; Cardiovascular System & Cardiology
语种英语
WOS记录号WOS:001168450800025
出版者LIPPINCOTT WILLIAMS & WILKINS
资助机构Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation
源URL[http://ir.ia.ac.cn/handle/173211/57952]  
专题模式识别国家重点实验室_计算生物学与机器智能
通讯作者Hao, Zhenhua; Yang, Ge; Li, Wei
作者单位1.Capital Med Univ, Beijing Childrens Hosp, 56 Nan Li Shi Rd, Beijing 100045, Peoples R China
2.Chinese Acad Sci, Inst Automat, 95 Zhong Guan Cun E Rd, Beijing 100190, Peoples R China
3.Beijing Pediat Res Inst, Beijing Key Lab Genet Birth Defects, Beijing, Peoples R China
4.Capital Med Univ, MOE Key Lab Major Dis Children, Beijing, Peoples R China
5.Capital Med Univ, Beijing Childrens Hosp, Natl Ctr Childrens Hlth, Beijing, Peoples R China
6.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing, Peoples R China
7.Chinese Acad Sci, Inst Genet & Dev Biol YW, State Key Lab Mol Dev Biol, Beijing, Peoples R China
8.Univ Chinese Acad Sci, Beijing, Peoples R China
9.Peking Univ, Inst Mol Med, Natl Biomed Imaging Ctr, Sch Future Technol,State Key Lab Membrane Biol,Be, Beijing, Peoples R China
10.Peking Univ, Biomed Engn Dept, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Jing,Hao, Zhenhua,Zhang, Yudong,et al. Physical Contacts Between Mitochondria and WPBs Participate in WPB Maturation[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,2024,44(1):108-123.
APA Ma, Jing.,Hao, Zhenhua.,Zhang, Yudong.,Li, Liuju.,Huang, Xiaoshuai.,...&Li, Wei.(2024).Physical Contacts Between Mitochondria and WPBs Participate in WPB Maturation.ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,44(1),108-123.
MLA Ma, Jing,et al."Physical Contacts Between Mitochondria and WPBs Participate in WPB Maturation".ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 44.1(2024):108-123.

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来源：自动化研究所

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