Tanreqing injection demonstrates anti-dengue activity through the regulation of the NF-κB-ICAM-1/VCAM-1 axis
文献类型：期刊论文
| 作者 | Yang, Jiabin2,3; Chen, Xi2,3; He, Xuemei2,3; Fang, Xiaochuan2,3; Liu, Shanhong2,3; Zou, Lifang2,3; Cao, Huihui2,3; Liu, Junshan2,3; Zuo, Jianping1 ; Yu, Linzhong2,3
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| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2024-07-25 |
| 卷号 | 130页码:15 |
| 关键词 | Tangreqing DENV-2 ICAM-1 VCAM-1 Dengue fever |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2024.155764 |
| 通讯作者 | Yu, Linzhong(yulzh@smu.edu.cn) ; Lu, Zibin(huang624@smu.edu.cn) |
| 英文摘要 | Background: Tanreqing injection (TRQ) has been employed in clinical practice as a treatment for dengue fever (DF). Nevertheless, the precise pharmacological mechanism underlying its efficacy remains elusive. Method: Network pharmacology, molecular docking, transcriptome sequencing, and experimental evaluation were employed to analyze and study the inhibitory potential of TRQ against dengue virus (DENV). Result: We found that TRQ inhibited the replication of DENV in human umbilical vein endothelial cells, Huh-7 cells, and Hep3B cells. In addition, TRQ prolonged the survival duration of AG129 mice infected with DF, decreased the viral load in serum and organs, and alleviated organ damage. Subsequently, ultra-highperformance liquid chromatography-tandem mass spectrometry analysis of TRQ was performed to identify 314 targets associated with 36 active compounds present in TRQ. Integration of multiple databases yielded 47 DF-related genes. Then, 15 hub targets of TRQ in DF were determined by calculating the network topology parameters (Degree). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that these pathways were primarily enriched in the processes of cytokine activation and leukocyte cross-endothelial migration, with significant enrichment of cell adhesion molecules. Molecular docking revealed favorable binding affinity between TRQ's key active compounds and the predicted hub targets. Transcriptome sequencing results showed TRQ's ability to restore the expression of vascular cell adhesion molecule-1 (VCAM-1) post-DENV infection. Finally, TRQ was found to modulate the immune status by regulating the nuclear factor kappa-B (NF kappa B)- intercellular cell adhesion molecule-1 (ICAM-1)/VCAM-1 axis, as well as reduce immune cell alterations, inflammatory factor secretion, vascular permeability, and bleeding tendencies induced by DENV infection. |
| WOS关键词 | ENDOTHELIAL-CELLS ; T-CELLS ; RESPONSES ; COMPONENTS ; CD4(+) |
| 资助项目 | National Natural Science Foundation of China[81730110] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001246487900001 |
| 出版者 | ELSEVIER GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/311422]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Yu, Linzhong; Lu, Zibin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Southern Med Univ, Sch Tradit Chinese Med, Guangdong Prov Key Lab Chinese Med Pharmaceut, Guangzhou 510515, Peoples R China 3.Southern Med Univ, Sch Tradit Chinese Med, Level Res Lab State Adm Tradit Chinese Med 3, Guangzhou 510515, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jiabin,Chen, Xi,He, Xuemei,et al. Tanreqing injection demonstrates anti-dengue activity through the regulation of the NF-κB-ICAM-1/VCAM-1 axis[J]. PHYTOMEDICINE,2024,130:15. |
| APA | Yang, Jiabin.,Chen, Xi.,He, Xuemei.,Fang, Xiaochuan.,Liu, Shanhong.,...&Lu, Zibin.(2024).Tanreqing injection demonstrates anti-dengue activity through the regulation of the NF-κB-ICAM-1/VCAM-1 axis.PHYTOMEDICINE,130,15. |
| MLA | Yang, Jiabin,et al."Tanreqing injection demonstrates anti-dengue activity through the regulation of the NF-κB-ICAM-1/VCAM-1 axis".PHYTOMEDICINE 130(2024):15. |
入库方式： OAI收割
来源：上海药物研究所
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