Imbalance between hippocampal projection cell and parvalbumin interneuron architecture increases epileptic susceptibility in mouse model of methyl CpG binding protein 2 duplication syndrome
文献类型：期刊论文
| 作者 | Ge, Junye5; Xie, Shengjun4; Duan, Jiamei3; Tian, Biqing5; Ren, Pengfei5; Hu, Erling5; Huang, Qiyi5; Mao, Honghui5; Zou, Yuxin5; Chen, Qian1,2,3 |
| 刊名 | EPILEPSIA
 |
| 出版日期 | 2024-05-31 |
| 页码 | 14 |
| 关键词 | autism epilepsy hippocampus MeCP2 parvalbumin |
| ISSN号 | 0013-9580 |
| DOI | 10.1111/epi.18027 |
| 通讯作者 | Chen, Qian(chenqian@zidd.ac.cn) ; Wang, Wenting(wwt0657@fmmu.edu.cn) |
| 英文摘要 | Objective: Methyl CpG-binding protein 2 (MECP2) duplication syndrome is a rare X-linked genomic disorder affecting predominantly males, which is usually manifested as epilepsy and autism spectrum disorder (ASD) comorbidity. The transgenic line MeCP2(Tg1) was used for mimicking MECP2 duplication syndrome and showed autism-epilepsy co-occurrence. Previous works suggested that the excitatory/inhibitory (E/I) imbalance is a potential common mechanism for both epilepsy and ASD. The projection neurons and parvalbumin (PV) interneurons account for the majority of E/I balance in the hippocampus. Therefore, we explored how structural changes of projection and PV+ neurons occur in the hippocampus of MeCP2(Tg1) mice and whether these morphological changes contribute to epilepsy susceptibility. Methods: We used the interneuron Designer receptors exclusively activated by designer drugs mouse model to inhibit inhibitory neurons in the hippocampus to verify the epilepsy susceptibility of MeCP2(Tg1) (FVB, an inbred strain named as sensitivity to Friend leukemia virus) mice. Electroencephalograms were recorded for the definition of seizure. We performed retro-orbital injection of virus in MeCP2(Tg1) (FVB):CaMKII alpha-Cre (C57BL/6) mice or MeCP2(Tg1):PV-Cre (C57BL/6) mice and their littermate controls to specifically label projection and PV+ neurons for structural analysis. Results: Epilepsy susceptibility was increased in MeCP2(Tg1) mice. There was a reduced number of PV neurons and reduced dendritic complexity in the hippocampus of MeCP2(Tg1) mice. The dendritic complexity in MeCP2(Tg1) mice was increased compared to wild-type mice, and total dendritic spine density in dentate gyrus of MeCP2(Tg1) mice was also increased. Total dendritic spine density was increased in CA1 of MeCP2(Tg1) mice. Significance: Overexpression of MeCP2 may disrupt crucial signaling pathways, resulting in decreased dendritic complexity of PV interneurons and increased dendritic spine density of projection neurons. This reciprocal modulation of excitatory and inhibitory neuronal structures associated with MeCP2 implies its significance as a potential target in the development of epilepsy and offers a novel perspective on the co-occurrence of autism and epilepsy. |
| WOS关键词 | LIMBIC SEIZURES ; MECP2 |
| 资助项目 | Guangdong province High-level Innovative Research Institute ; Natural Science Foundation of China[82071536] ; Natural Science Foundation of China[81771476] ; Natural Science Foundation of China[81871078] ; Natural Science Foundation of China[82271578] ; Shaanxi Province Key Industrial Innovation Chain Project[2023-ZDLSF-47] ; Shanghai Pujiang Program[22PJ1415500] ; Zhongshan Municipal Bureau of Science and Technology[CXTD2022013] ; [2021B0909050003] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:001236338700001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/311782]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Qian; Wang, Wenting |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan, Peoples R China 3.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China 4.Yangtze Univ, Jingzhou Hosp, Jingzhou, Peoples R China 5.Fourth Mil Med Univ, Sch Basic Med, Dept Neurobiol, Xian 710032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Junye,Xie, Shengjun,Duan, Jiamei,et al. Imbalance between hippocampal projection cell and parvalbumin interneuron architecture increases epileptic susceptibility in mouse model of methyl CpG binding protein 2 duplication syndrome[J]. EPILEPSIA,2024:14. |
| APA | Ge, Junye.,Xie, Shengjun.,Duan, Jiamei.,Tian, Biqing.,Ren, Pengfei.,...&Wang, Wenting.(2024).Imbalance between hippocampal projection cell and parvalbumin interneuron architecture increases epileptic susceptibility in mouse model of methyl CpG binding protein 2 duplication syndrome.EPILEPSIA,14. |
| MLA | Ge, Junye,et al."Imbalance between hippocampal projection cell and parvalbumin interneuron architecture increases epileptic susceptibility in mouse model of methyl CpG binding protein 2 duplication syndrome".EPILEPSIA (2024):14. |
入库方式： OAI收割
来源：上海药物研究所
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