Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds
文献类型：期刊论文
| 作者 | Lin,Jia-Ling; Fang,Xin; Li,Jian-Xu; Chen,Zhi-Wen; Wu,Wen-Kai; Guo,Xiao-Xiang; Liu,Ning-Jing; Huang,Jia-Fa; Chen,Fang-Yan; Wang,Ling-Jian |
| 刊名 | NATURE PLANTS
 |
| 出版日期 | 2023 |
| 卷号 | 9期号:4页码:605-+ |
| 关键词 | DOMAIN-CONTAINING PROTEIN BIOSYNTHESIS (-)-GOSSYPOL SYNTHASE (+)-GOSSYPOL METABOLISM EXPRESSION REDUCTION |
| ISSN号 | 2055-0278 |
| DOI | 10.1038/s41477-023-01376-2 |
| 英文摘要 | This study identified two dirigent proteins responsible for the biosynthesis of the two enantiomers of gossypol in cotton. Editing one of the dirigent proteins selectively removed the toxic (-)-gossypol in cotton seeds without affecting pest resistance. Axial chirality of biaryls can generate varied bioactivities. Gossypol is a binaphthyl compound made by cotton plants. Of its two axially chiral isomers, (-)-gossypol is the bioactive form in mammals and has antispermatogenic activity, and its accumulation in cotton seeds poses health concerns. Here we identified two extracellular dirigent proteins (DIRs) from Gossypium hirsutum, GhDIR5 and GhDIR6, which impart the hemigossypol oxidative coupling into (-)- and (+)-gossypol, respectively. To reduce cotton seed toxicity, we disrupted GhDIR5 by genome editing, which eliminated (-)-gossypol but had no effects on other phytoalexins, including (+)-gossypol, that provide pest resistance. Reciprocal mutagenesis identified three residues responsible for enantioselectivity. The (-)-gossypol-forming DIRs emerged later than their enantiocomplementary counterparts, from tandem gene duplications that occurred shortly after the cotton genus diverged. Our study offers insight into how plants control enantiomeric ratios and how to selectively modify the chemical spectra of cotton plants and thereby improve crop quality. |
| 学科主题 | Plant Sciences |
| WOS记录号 | WOS:000950522700004 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75420]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Lin,Jia-Ling,Fang,Xin,Li,Jian-Xu,et al. Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds[J]. NATURE PLANTS,2023,9(4):605-+. |
| APA | Lin,Jia-Ling.,Fang,Xin.,Li,Jian-Xu.,Chen,Zhi-Wen.,Wu,Wen-Kai.,...&Huang,Jin-Quan.(2023).Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds.NATURE PLANTS,9(4),605-+. |
| MLA | Lin,Jia-Ling,et al."Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds".NATURE PLANTS 9.4(2023):605-+. |
入库方式： OAI收割
来源：昆明植物研究所
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