Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry
文献类型：期刊论文
| 作者 | Qin,Yan; He,Ying-Jie; Zhao,Yun-Li; Zhou,Zhong-Shun; Wang,Zhao-Jie; Zhu,Yan-Yan; Luo,Xiao-Dong
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2023 |
| 关键词 | CATHARANTHUS-ROSEUS ETHANOLIC EXTRACTS IDENTIFICATION QUANTIFICATION HPTLC |
| ISSN号 | 1615-9314 |
| DOI | 10.1002/jssc.202200843 |
| 英文摘要 | Monoterpene indole alkaloids exhibit structural diversity in herbal resources and have been developed as promising drugs owing to their significant biological activities. Confidential identification and quantification of monoterpene indole alkaloids is the key to quality control of target plants in industrial production but has rarely been reported. In this study, quantitative performance of three data acquisition modes of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry including full scan, auto-MS2 and target-MS2, was evaluated and compared for specificity, sensitivity, linearity, precision, accuracy, and matrix effect using five monoterpene indole alkaloids (scholaricine, 19-epi-scholaricine, vallesamine, picrinine, and picralinal). Method validations indicated that target-MS2 mode showed predominant performance for simultaneous annotation and quantification of analytes, and was then applied to determine monoterpene indole alkaloids in Alstonia scholaris (leaves, barks) after extraction procedures optimization using Box-Behnken design of response surface methodology. The variations of A. scholaris monoterpene indole alkaloids in different plant parts, harvest periods, and post-handling processes, were subsequently investigated. The results indicated that target-MS2 mode could improve the quantitative capability of ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for structure-complex monoterpene indole alkaloids in herbal matrices.Alstonia scholaris, monoterpene indole alkaloids, quadrupole time of flight mass spectrometry, qualitative and quantitative analysis, ultra-high-performance liquid chromatography |
| 学科主题 | Chemistry |
| WOS记录号 | WOS:001014416200001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75441]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Qin,Yan,He,Ying-Jie,Zhao,Yun-Li,et al. Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2023. |
| APA | Qin,Yan.,He,Ying-Jie.,Zhao,Yun-Li.,Zhou,Zhong-Shun.,Wang,Zhao-Jie.,...&Luo,Xiao-Dong.(2023).Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry.JOURNAL OF SEPARATION SCIENCE. |
| MLA | Qin,Yan,et al."Targeted quantitative analysis of monoterpenoid indole alkaloids in Alstonia scholaris by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry".JOURNAL OF SEPARATION SCIENCE (2023). |
入库方式： OAI收割
来源：昆明植物研究所
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