Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum
文献类型：期刊论文
| 作者 | Li,Desen; Yang,Minjie; Mu,Rongfang; Luo,Shihong ; Chen,Yuegui; Li,Wenyuan; Wang,An; Guo,Kai; Liu,Yan; Li,Shenghong
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| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2023 |
| 卷号 | 34期号:1页码:107469 |
| 关键词 | Sesterterpenoids Chimeric sesterterpene synthases Plant symbiotic fungi Alternaria alternata Leucosceptrum canum Anti-adipogenic activity GLANDULAR TRICHOMES ALTERNARIA-ALTERNATA HARBOR |
| ISSN号 | 1878-5964 |
| DOI | 10.1016/j.cclet.2022.04.067 |
| 英文摘要 | Two chimeric sesterterpene synthases (AaTPS1 and AaTPS2) were functionally characterized from Alternaria alternata MB-30 isolated from the leaves of a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum. AaTPS1 generated a 5/8/6/5 tetracyclic sesteraltererol (1) and its absolute stereochemistry was determined by X-ray crystallographic analysis of its derivative 10,11-epoxysesteraltererol (2), which enabled revision of the absolute configuration of C7 of sesterfisherol produced by NfSS and PTTS014 characterized previously and its derivative 10,11-epoxysesterfisherol. AaTPS2 produced a 5/15 bicyclic preterpestacin I (3). Site-directed mutagenesis suggested that F192 in AaTPS1 was likely involved in controlling of the hydroxylation of C12, and eight amino acids were important for the enzyme activity of AaTPS1 and AaTPS2. The engineered Escherichia coli and Saccharomyces cerevisiae strains were constructed for the productions of compounds 1 and 3, and the highest titer of compound 1 reached 62.3 mg/L in shake-flask culture. Both compounds 1 and 2 showed anti-adipogenic activity. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. |
| 学科主题 | Chemistry |
| WOS记录号 | WOS:000893634100027 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75457]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Li,Desen,Yang,Minjie,Mu,Rongfang,et al. Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum[J]. CHINESE CHEMICAL LETTERS,2023,34(1):107469. |
| APA | Li,Desen.,Yang,Minjie.,Mu,Rongfang.,Luo,Shihong.,Chen,Yuegui.,...&Li,Shenghong.(2023).Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum.CHINESE CHEMICAL LETTERS,34(1),107469. |
| MLA | Li,Desen,et al."Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum".CHINESE CHEMICAL LETTERS 34.1(2023):107469. |
入库方式： OAI收割
来源：昆明植物研究所
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