Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis
文献类型：期刊论文
| 作者 | Tuo,Xiaotao; Yu,Zhiyin; Li,Junheng; Qi,Yuxin; Peng,Guoqing; Huang,Sheng-Xiong; Huang,Xueshuang; Huang,Jian-Ping |
| 刊名 | JOURNAL OF PLANT PHYSIOLOGY
 |
| 出版日期 | 2023 |
| 卷号 | 285页码:153983 |
| 关键词 | Aristolochia Aristolochic acids Biosynthetic pathway Transcriptome Methyltransferase BENZYLISOQUINOLINE ALKALOID BIOSYNTHESIS RATE-LIMITING STEP NORCOCLAURINE 6-O-METHYLTRANSFERASE PURIFICATION |
| ISSN号 | 1618-1328 |
| DOI | 10.1016/j.jplph.2023.153983 |
| 英文摘要 | In view of the nephrotoxicity, hepatotoxicity, and carcinogenicity of aristolochic acids (AAs), the removal of AAs from plants becomes an urgent priority for ensuring the safety of Aristolochia herbal materials. In this study, based on the root-predominant distribution of aristolochic acid I (AAI) in Aristolochia debilis, transcriptome sequencing, in combination with phylogenetic analyses, and gene expression pattern analysis together provided five candidate genes for investigating AAI biosynthesis. Comprehensive in vitro and in vivo enzymatic assays revealed that Ab6OMT1 (6-O-methyltransferase) and AbNMT1 (N-methyltransferase) exhibit promiscuity in substrate recognition, and they could act in a cooperative fashion to achieve conversion of norlaudanosoline, a predicted intermediate in AAI biosynthetic route, into 3'-hydroxy-N-methylcoclaurine through two different methylation reaction sequences. These results shed light on the molecular basis for AAI biosynthesis in Aristo-lochia herbs. More importantly, Ab6OMT1 and AbNMT1 may be employed as targets for the metabolic engi-neering of AAI biosynthesis to produce AAs-free Aristolochia herbal materials. |
| 学科主题 | Plant Sciences |
| WOS记录号 | WOS:000992644700001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75466]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Tuo,Xiaotao,Yu,Zhiyin,Li,Junheng,et al. Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis[J]. JOURNAL OF PLANT PHYSIOLOGY,2023,285:153983. |
| APA | Tuo,Xiaotao.,Yu,Zhiyin.,Li,Junheng.,Qi,Yuxin.,Peng,Guoqing.,...&Huang,Jian-Ping.(2023).Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis.JOURNAL OF PLANT PHYSIOLOGY,285,153983. |
| MLA | Tuo,Xiaotao,et al."Characterization of two putative norlaudanosoline methyltransferases from Aristolochia debilis".JOURNAL OF PLANT PHYSIOLOGY 285(2023):153983. |
入库方式： OAI收割
来源：昆明植物研究所
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