A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities
文献类型：期刊论文
| 作者 | Li,De-Sen; Shi,Lin-Lin; Guo,Kai; Luo,Shi-Hong; Liu,Yan-Chun; Chen,Yue-Gui; Liu,Yan; Li,Sheng-Hong
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| 刊名 | PHYTOCHEMISTRY
 |
| 出版日期 | 2023 |
| 卷号 | 211页码:113681 |
| 关键词 | Sesquiterpenoids Sesquiterpene synthases (R)-fi-bisabolene Colquhounia coccinea var mollis Anti-adipogenic activity GERANYLFARNESYL DIPHOSPHATE SYNTHASE GLANDULAR TRICHOMES FUNCTIONAL EXPRESSION BETA-BISABOLENE ESSENTIAL OILS BIOSYNTHESIS IDENTIFICATION CYTOTOXICITY CLONING |
| ISSN号 | 1873-3700 |
| DOI | 10.1016/j.phytochem.2023.113681 |
| 英文摘要 | The sesquiterpene fi-bisabolene possessing R and S configurations is commonly found in plant essential oils with antimicrobial and antioxidant activities. Here, we report the cloning and functional characterization of a (R)-fi-bisabolene synthase gene (CcTPS2) from a Lamiaceae medicinal plant Colquhounia coccinea var. mollis. The biochemical function of CcTPS2 catalyzing the cyclization of farnesyl diphosphate to form a single product (R)-fi-bisabolene was characterized through an engineered Escherichia coli producing diverse polyprenyl diphosphate precursors and in vitro enzyme assay, indicating that CcTPS2 was a high-fidelity (R)-fi-bisabolene synthase. The production of (R)-fi-bisabolene in an engineered E. coli strain harboring the exogenous mevalonate pathway, farnesyl diphosphate synthase and CcTPS1 genes was 17 mg/L under shaking flask conditions. Ultimately, 120 mg of purified (R)-fi-bisabolene was obtained from the engineered E. coli, and its structure was elucidated by detailed spectroscopic analyses (including 1D and 2D NMR, and specific rotation). Four chimeric enzymes were constructed through domain swapping, which altered the product outcome, indicating the region important for substrate and product specificity. In addition, (R)-fi-bisabolene exhibited anti-adipogenic activity in the model organism Caenorhabditis elegans and antibacterial activity selectively against Gram-positive bacteria. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000988873400001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75529]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Li,De-Sen,Shi,Lin-Lin,Guo,Kai,et al. A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities[J]. PHYTOCHEMISTRY,2023,211:113681. |
| APA | Li,De-Sen.,Shi,Lin-Lin.,Guo,Kai.,Luo,Shi-Hong.,Liu,Yan-Chun.,...&Li,Sheng-Hong.(2023).A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities.PHYTOCHEMISTRY,211,113681. |
| MLA | Li,De-Sen,et al."A new sesquiterpene synthase catalyzing the formation of (R)-fi-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities".PHYTOCHEMISTRY 211(2023):113681. |
入库方式： OAI收割
来源：昆明植物研究所
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