Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress
文献类型：期刊论文
| 作者 | Chen,Yonghong; Yang,Xiaoyu; Zhang,Longlong; Wu,Qunfu; Li,Shuhong; Gou,Jianghui; He,Jiangbo; Zhang,Keqin; Li,Shenghong ; Niu,Xuemei
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| 刊名 | HELIYON
 |
| 出版日期 | 2023 |
| 卷号 | 9期号:2页码:e13066 |
| 关键词 | Thermomyces dupontii Prenylated indole alkaloids Tryptophan Lipids Cold stress Tryptophan metabolism PRENYLATED INDOLE ALKALOIDS SECONDARY METABOLISM THERMOPHILIC FUNGI PEROXIDATION DIVERSITY |
| ISSN号 | 2405-8440 |
| DOI | 10.1016/j.heliyon.2023.e13066 |
| 英文摘要 | Tryptophan and its derived metabolites have been assumed to play important roles in the development and survival of organisms. However, the links of tryptophan and its derived metabolites to temperature change remained largely cryptic. Here we presented that a class of prenyl indole alkaloids biosynthesized from tryptophan dramatically accumulated in thermophilic fungus Thermomyces dupontii under cold stress, in which lipid droplets were also highly accumulated and whose conidiophores were highly build-up. Concurrently, disruption of the key NRPS gene involved in the biosynthesis of prenyl indole alkaloids, resulted in decreased lipid and shrunken mitochondria but enlarged vacuoles. Moreover, the Fe3+ and superoxide levels in Delta NRPS were significantly increased but the reactive oxygen species lipid peroxidation and autophagy levels decreased. Metabolomics study revealed that most enriched metabolites in Delta NRPS were mainly composed of tryptophan degraded metabolites including well known ROS scavenger kynurenamines, and lipid-inhibitors, anthranilic acid and indoleacetic acid, and free radical reaction suppressor free fatty acids. Transcriptomic analysis suggested that the key gene involved in tryptophan metabolism, coinciding with the lipid metabolic processes and ion transports were most up-regulated in Delta NRPS under stress. Our results confirmed a lipid-mediated fungal response to cold stress and unveiled a link of tryptophan-based metabolic reprogramming to the fungal cold adaption. |
| 学科主题 | Science & Technology - Other Topics |
| WOS记录号 | WOS:000969447800001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75556]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Chen,Yonghong,Yang,Xiaoyu,Zhang,Longlong,et al. Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress[J]. HELIYON,2023,9(2):e13066. |
| APA | Chen,Yonghong.,Yang,Xiaoyu.,Zhang,Longlong.,Wu,Qunfu.,Li,Shuhong.,...&Niu,Xuemei.(2023).Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress.HELIYON,9(2),e13066. |
| MLA | Chen,Yonghong,et al."Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stress".HELIYON 9.2(2023):e13066. |
入库方式： OAI收割
来源：昆明植物研究所
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