Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota
文献类型：期刊论文
| 作者 | Zuo,Zhichuang; He,Zhicheng; Ma,Yan; Xu,Chen; Tao,Xuelin; Ning,Zimo; Gao,Li; Rehemiding,Ailainuer; Gao,Na; Zhao,Jinhua
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2023 |
| 卷号 | 236页码:123935 |
| 关键词 | Slug Galactan Chemical structure Oligosaccharide Human gut microbiota SULFATED GALACTANS HELIX-POMATIA POLYSACCHARIDES BIOACTIVITY HYDROLYSIS |
| ISSN号 | 1879-0003 |
| DOI | 10.1016/j.ijbiomac.2023.123935 |
| 英文摘要 | The slug Vaginulus alte is used as folk medicine in China, but the structure and activities of its galactan com-ponents remain to be clarified. Here, the galactan from V. alte (VAG) was purified. The Mw of VAG was determined as-28.8 kDa. Chemical composition analysis showed that VAG was composed of D-galactose (75 %) and L-galactose (25 %). To elucidate its precise structure, a series of disaccharides and trisaccharides were pu-rified from mild acid hydrolyzed VAG and their structures were characterized by 1D/2D NMR spectroscopy. Based on methylation analysis and structural analysis of oligosaccharides, VAG was elucidated as a highly branched polysaccharide and mainly composed of (1 -> 6)-or (1 -> 3)-linked beta-D-galactose, and distinct (1 -> 2)-linked alpha-L-galactose. The investigation of probiotic effects in vitro revealed that VAG could promote the growth of B. thetaiotaomicron and B. ovatus, while had no effect on the growth of L. acidophilus, L. rhamnosus, B. longum subsp. infantis and B. animalis subsp. lactis, but dVAG-3 with Mw-1.0 kDa could promote the growth of L. acidophilus. These results will provide insights into specific structures and functions of polysaccharides from the V. alte. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS记录号 | WOS:000959437500001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75579]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Zuo,Zhichuang,He,Zhicheng,Ma,Yan,et al. Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2023,236:123935. |
| APA | Zuo,Zhichuang.,He,Zhicheng.,Ma,Yan.,Xu,Chen.,Tao,Xuelin.,...&Zhao,Jinhua.(2023).Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,236,123935. |
| MLA | Zuo,Zhichuang,et al."Structure characterization of a highly branched galactan from the slug Vaginulus alte and its utilization by human gut microbiota".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 236(2023):123935. |
入库方式： OAI收割
来源：昆明植物研究所
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