Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms
文献类型：期刊论文
| 作者 | J. Wang, L. Cong, W. Shi, W. Xu and S. Xu |
| 刊名 | Analytical Chemistry
 |
| 出版日期 | 2023 |
| 卷号 | 95期号:29页码:11019-11027 |
| ISSN号 | 00032700 |
| DOI | 10.1021/acs.analchem.3c01273 |
| 英文摘要 | A microdroplet-based surface-enhanced Raman spectroscopy (microdroplet SERS) platform was constructed to envelop individual cells in microdroplets, followed by the SERS detection of their extracellular vesicle-proteins (EV-proteins) via the in-drop immunoassays by use of immunomagnetic beads (iMBs) and immuno-SERS tags (iSERS tags). A unique phenomenon is found that iMBs can start a spontaneous reorientation on the probed cell surface based on the electrostatic force-driven interfacial aggregation effect, which leads EV-proteins and iSERS tags to be gathered from a liquid phase to a cell membrane interface and significantly improves SERS sensitivity to the single-cell analysis level due to the formation of numbers of SERS hotspots. Three EV-proteins from two breast cancer cell lines were collected and further analyzed by machine learning algorithmic tools, which will be helpful for a deeper understanding of breast cancer subtypes from the view of EV-proteins. © 2023 American Chemical Society. |
| URL标识 | 查看原文 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/67934]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | J. Wang, L. Cong, W. Shi, W. Xu and S. Xu. Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms[J]. Analytical Chemistry,2023,95(29):11019-11027. |
| APA | J. Wang, L. Cong, W. Shi, W. Xu and S. Xu.(2023).Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms.Analytical Chemistry,95(29),11019-11027. |
| MLA | J. Wang, L. Cong, W. Shi, W. Xu and S. Xu."Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms".Analytical Chemistry 95.29(2023):11019-11027. |
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