Raman identification of single cell component and FMS-like tyrosine kinase 3-internal tandem duplications subtype for clinical acute myeloid leukemia
文献类型：期刊论文
| 作者 | Y. Wang, Y. Wu, M. Chi, M. Li, S. Gao, L. Su and W. Han |
| 刊名 | Journal of Raman Spectroscopy
 |
| 出版日期 | 2023 |
| 卷号 | 54期号:6页码:596-607 |
| ISSN号 | 03770486 |
| DOI | 10.1002/jrs.6523 |
| 英文摘要 | Acute myeloid leukemia (AML) is a malignant clonal blood disease of hematopoietic stem cells with poor prognosis. Traditional diagnosis of AML depends on the morphology, immunology, cytogenetics, and molecular biology (MICM) classification. As a rapid, label-free and non-destructive detection method, Raman spectroscopy can characterize the molecular information in the biochemical process at the molecular level through peak position, intensity and other information. In this paper, we collected leukemic blast cells from 19 AML patients. Firstly, it was proved that with the help of multivariate analysis methods such as principal component analysis-linear discriminant analysis (PCA-LDA) and multivariate curve resolution-alternating least squares (MCR-ALS), Raman spectroscopy could effectively distinguish AML cells from normal leukocytes, and the accuracy rate was up to 96.71%. The results showed that compared with normal leukocytes, multiple components of information decomposed by the AML spectrum represented abnormal alterations in proteins, nucleic acids, lipids and carbohydrates in leukemia cells. Secondly, the same procedure was used to further detect different types of AML and found that Raman spectroscopy could distinguish AML cells with FMS-like tyrosine kinase 3-Internal Tandem Duplications (FLT3-ITD) mutations or not, and there were differences in spectral characteristics corresponding to nucleic acids and proteins (amino acids). The above results revealed that Raman spectroscopy has great potential for clinical diagnosis and mechanistic study of AML. © 2023 John Wiley & Sons Ltd. |
| URL标识 | 查看原文 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/67997]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | Y. Wang, Y. Wu, M. Chi, M. Li, S. Gao, L. Su and W. Han. Raman identification of single cell component and FMS-like tyrosine kinase 3-internal tandem duplications subtype for clinical acute myeloid leukemia[J]. Journal of Raman Spectroscopy,2023,54(6):596-607. |
| APA | Y. Wang, Y. Wu, M. Chi, M. Li, S. Gao, L. Su and W. Han.(2023).Raman identification of single cell component and FMS-like tyrosine kinase 3-internal tandem duplications subtype for clinical acute myeloid leukemia.Journal of Raman Spectroscopy,54(6),596-607. |
| MLA | Y. Wang, Y. Wu, M. Chi, M. Li, S. Gao, L. Su and W. Han."Raman identification of single cell component and FMS-like tyrosine kinase 3-internal tandem duplications subtype for clinical acute myeloid leukemia".Journal of Raman Spectroscopy 54.6(2023):596-607. |
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