COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS
文献类型：期刊论文
| 作者 | Diantina,Surya; McGill,Craig; McCormick,Andrea Clavijo; Millner,James; Pritchard,Hugh W.; Nadarajan,Jayanthi |
| 刊名 | CRYOLETTERS
 |
| 出版日期 | 2023 |
| 卷号 | 44期号:4页码:197-207 |
| 关键词 | cryobiotechnology ex situ conservation direct cooling seed storage temperate species tropical species vitrification CONSERVATION GERMINATION LONGEVITY STORAGE CRYOBIOTECHNOLOGY PROTOCOLS |
| ISSN号 | 1742-0644 |
| DOI | 10.54680/fr23410110312 |
| 英文摘要 | BACKGROUND: The atypical seed storage behaviour reported in several orchid species justifies cryopreservation as a complementary conservation strategy to conventional seed banking. OBJECTIVE: This study aimed to assess the seed cryopreservation potential of five orchid species; two tropical epiphytic, Indonesian species (Dendrobium strebloceras, D. lineale), one temperate epiphytic, New Zealand species (D. cunninghamii) and two temperate terrestrial, New Zealand species (Pterostylis banksii, Thelymitra nervosa). MATERIALS AND METHODS: Seeds were cryopreserved by direct immersion in liquid nitrogen (LN) and through the application of a cryoprotectant vitrification method. For the latter, seeds were exposed to Plant Vitrification Solution 2 (PVS2) for 0, 20, 50, and 70 min, at either room temperature or on ice, prior to immersion in LN. RESULTS: Seeds of all the studied species germinated well following direct cooling in LN. There was no difference in the seedling development capability between cryopreserved and non-cryopreserved seeds of both tropical epiphytic species and direct immersion in LN enhanced seed germination and shoot formation in both temperate terrestrials. CONCLUSION: Through a range of analyses of germination and postgermination growth, our study shows the potential for cryopreserving epiphytic or terrestrial orchids from tropical and temperate regions. |
| WOS记录号 | WOS:001167715500004 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/75619]  |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Diantina,Surya,McGill,Craig,McCormick,Andrea Clavijo,et al. COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS[J]. CRYOLETTERS,2023,44(4):197-207. |
| APA | Diantina,Surya,McGill,Craig,McCormick,Andrea Clavijo,Millner,James,Pritchard,Hugh W.,&Nadarajan,Jayanthi.(2023).COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS.CRYOLETTERS,44(4),197-207. |
| MLA | Diantina,Surya,et al."COMPARATIVE SEED CRYOPRESERVATION OF INDONESIAN AND NEW ZEALAND EPIPHYTIC AND TERRESTRIAL ORCHIDS".CRYOLETTERS 44.4(2023):197-207. |
入库方式： OAI收割
来源：昆明植物研究所
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