Bond-selective intensity diffraction tomography
文献类型：期刊论文
| 作者 | J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng |
| 刊名 | Nature Communications
 |
| 出版日期 | 2022 |
| 卷号 | 13期号:1页码:12 |
| DOI | 10.1038/s41467-022-35329-8 |
| 英文摘要 | Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of similar to 20s per volume, with a lateral and axial resolution of similar to 350nm and similar to 1.1 mu m, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (similar to 100 mu m x 100 mu m). The authors introduce Bond-selective Intensity Diffraction Tomography, a computational mid-infrared photothermal microscopy technique based on a standard bright-field microscope and an add-on pulsed light source. It recovers both mid-infrared spectra and bond-selective 3D refractive index maps based on intensity-only measurements. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/68231]  |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng. Bond-selective intensity diffraction tomography[J]. Nature Communications,2022,13(1):12. |
| APA | J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng.(2022).Bond-selective intensity diffraction tomography.Nature Communications,13(1),12. |
| MLA | J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng."Bond-selective intensity diffraction tomography".Nature Communications 13.1(2022):12. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。