GFPBW1, a β-glucan from Grifola frondosa as vaccine adjuvant: APCs activation and maturation
文献类型：期刊论文
| 作者 | He, Xiang4; Lu, Jiang-ling1,2,3; Liao, Wen-feng2,3; Long, Yi-ru2,3; Zhang, Xing4; Zhu, Qian3; Lu, Heng-lei3; Hao, Geng-yan2,3; Ding, Kan1,2,3 ; Sun, Jian-hua2,3
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2024-06-21 |
| 页码 | 11 |
| 关键词 | cancer vaccine adjuvant granola frondose polysaccharide GFPBW1 beta-glucan |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/s41401-024-01330-8 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) ; Sun, Jian-hua(jhsun@cdser.simm.ac.cn) ; Gong, Li-kun(lkgong@cdser.simm.ac.cn) ; Yang, Yi-fu(0000002402@shutcm.edu.cn) |
| 英文摘要 | Adjuvants for vaccines with characteristics of improving adaptive immunity particularly via leverage of antigen presenting cells (APCs) are currently lacking. In a previous work we obtained a new soluble 300 kDa homogeneous beta-glucan named GFPBW1 from the fruit bodies of Granola frondosa. GFPBW1 could activate macrophages by targeting dendritic cell associated C-type lectin 1 (Dectin-1)/Syk/NF-kappa B signaling to achieve antitumour effects. In this study the adjuvant effects of GFPBW1 were explored with OVA-antigen and B16-OVA tumor model. We showed that GFPBW1 (5, 50, 500 mu g/mL) dose-dependently promoted activation and maturation of APCs in vitro by increasing CD80, CD86 and MHC II expression. We immunized female mice with OVA in combination with GFPBW1 (50 or 300 mu g) twice with an interval of two weeks. GFPBW1 markedly and dose-dependently increased OVA-specific antibody titers of different subtypes including IgG1, IgG2a, IgG2b and IgG3, suggesting that it could serve as an adjuvant for both Th1 and Th2 type immune responses. Furthermore, GFPBW1 in combination with aluminum significantly increased the titers of OVA-specific IgG2a and IgG2b, but not those of IgG1, suggesting that GFPBW1 could be used as a co-adjuvant of aluminum to compensate for Th1 deficiency. For mice immunized with OVA plus GFPBW1, no obvious pathological injury was observed in either major organs or injection sites, and no abnormalities were noted for any of the hematological parameters. When GFPBW1 served as an adjuvant in the B16-OVA cancer vaccine models, it could accomplish entire tumor suppression with preventive vaccines, and enhance antitumour efficacy with therapeutic vaccines. Differentially expressed genes were found to be enriched in antigen processing process, specifically increased tumor infiltration of DCs, B1 cells and plasma cells in the OVA plus GFPBW1 group, in accordance with its activation and maturation function of APCs. Collectively, this study systematically describes the properties of GFPBW1 as a novel potent and safe adjuvant and highlights its great potential in vaccine development. |
| WOS关键词 | DECTIN-1 ; POLYSACCHARIDES ; RECOGNITION |
| 资助项目 | National Natural Science Foundation of China[82341039] ; National Key Research and Development Plan[2022YFC2304105] ; Shanghai Municipal Science and Technology Major Project ; Science and Technology Innovation Action Plan of Shanghai[22S11902100] ; Science and Technology Innovation Action Plan of Shanghai[22140900500] ; National Science Foundation for Distinguished Young Scholars of China[32271332] ; Zhongshan Municipal Bureau of Science and Technology[CXTD2023010] ; Department of Science and Technology of Guangdong Province (High-level Innovative Research Institute)[2021B0909050003] ; Shanghai Institute of Materia Medica |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001252348800001 |
| 出版者 | NATURE PUBL GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/311914]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Ding, Kan; Sun, Jian-hua; Gong, Li-kun; Yang, Yi-fu |
| 作者单位 | 1.Chinese Acad Sci, Inst Drug Discovery & Dev, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Expt Ctr Sci & Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xiang,Lu, Jiang-ling,Liao, Wen-feng,et al. GFPBW1, a β-glucan from Grifola frondosa as vaccine adjuvant: APCs activation and maturation[J]. ACTA PHARMACOLOGICA SINICA,2024:11. |
| APA | He, Xiang.,Lu, Jiang-ling.,Liao, Wen-feng.,Long, Yi-ru.,Zhang, Xing.,...&Yang, Yi-fu.(2024).GFPBW1, a β-glucan from Grifola frondosa as vaccine adjuvant: APCs activation and maturation.ACTA PHARMACOLOGICA SINICA,11. |
| MLA | He, Xiang,et al."GFPBW1, a β-glucan from Grifola frondosa as vaccine adjuvant: APCs activation and maturation".ACTA PHARMACOLOGICA SINICA (2024):11. |
入库方式： OAI收割
来源：上海药物研究所
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