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Superposition of Substrate Deformation Fields Induced by Molecular Clutches Explains Cell Spatial Sensing of Ligands

文献类型：期刊论文

作者Xue, Ruihao2; Chen, Yonggang2; Gong, Ze1,2; Jiang, Hongyuan2
刊名ACS NANO
出版日期2024-08-01
卷号18期号:32页码:21144-21155
关键词adhesion dynamics ligand spacing cell spreading motor-clutch model spatial sensing
ISSN号1936-0851
DOI10.1021/acsnano.4c03667
通讯作者Gong, Ze(gongze@ustc.edu.cn) ; Jiang, Hongyuan(jianghy@ustc.edu.cn)
英文摘要Cells can sense the physical properties of the extracellular matrices (ECMs), such as stiffness and ligand density, through cell adhesions to actively regulate their behaviors. Recent studies have shown that varying ligand spacing of ECMs can influence adhesion size, cell spreading, and even stem cell differentiation, indicating that cells have the spatial sensing ability of ECM ligands. However, the mechanism of the cells' spatial sensing remains unclear. In this study, we have developed a lattice-spring motor-clutch model by integrating cell membrane deformation, the talin unfolding mechanism, and the lattice spring for substrate ligand distribution to explore how the spatial distribution of integrin ligands and substrate stiffness influence cell spreading and adhesion dynamics. By applying the Gillespie algorithm, we found that large ligand spacing reduces the superposition effect of the substrate's displacement fields generated by pulling force from motor-clutch units, increasing the effective stiffness probed by the force-sensitive receptors; this finding explains a series of previous experiments. Furthermore, using the mean-field theory, we obtain the effective stiffness sensed by bound clutches analytically; our analysis shows that the bound clutch number and ligand spacing are the two key factors that affect the superposition effects of deformation fields and, hence, the effective stiffness. Overall, our study reveals the mechanism of cells' spatial sensing, i.e., ligand spacing changes the effective stiffness sensed by cells due to the superposition effect of deformation fields, which provides a physical clue for designing and developing biological materials that effectively control cell behavior and function.
分类号一类
WOS关键词FORCE TRANSMISSION ; MATRIX ; DYNAMICS ; MODEL ; TRACTION ; ADHESION ; STIFFNESS ; MASTER ; TALIN
资助项目National Natural Science Foundation of China[12202439] ; National Natural Science Foundation of China[12025207] ; National Natural Science Foundation of China[11872357] ; USTC Research Funds of the Double First-Class Initiative[YD2090002012] ; Fundamental Research Funds for the Central Universities ; University of Science and Technology of China Center for Micro and Nanoscale Research and Fabrication
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001284877600001
资助机构National Natural Science Foundation of China ; USTC Research Funds of the Double First-Class Initiative ; Fundamental Research Funds for the Central Universities ; University of Science and Technology of China Center for Micro and Nanoscale Research and Fabrication
其他责任者Gong, Ze ; Jiang, Hongyuan
源URL[http://dspace.imech.ac.cn/handle/311007/96277]  
专题力学研究所_非线性力学国家重点实验室
作者单位1.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China
2.Univ Sci & Technol China, Dept Modern Mech, CAS Key Lab Mech Behav & Design Mat, Hefei 230027, Anhui, Peoples R China;
推荐引用方式
GB/T 7714		 Xue, Ruihao,Chen, Yonggang,Gong, Ze,et al. Superposition of Substrate Deformation Fields Induced by Molecular Clutches Explains Cell Spatial Sensing of Ligands[J]. ACS NANO,2024,18(32):21144-21155.
APA Xue, Ruihao,Chen, Yonggang,Gong, Ze,&Jiang, Hongyuan.(2024).Superposition of Substrate Deformation Fields Induced by Molecular Clutches Explains Cell Spatial Sensing of Ligands.ACS NANO,18(32),21144-21155.
MLA Xue, Ruihao,et al."Superposition of Substrate Deformation Fields Induced by Molecular Clutches Explains Cell Spatial Sensing of Ligands".ACS NANO 18.32(2024):21144-21155.

入库方式： OAI收割

来源：力学研究所

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