This Letter demonstrates the use of long-lifetime NO fluorescence for molecular tagging velocimetry (MTV) with (1 + 1) resonant-enhanced multiphoton ionization (REMPI) scheme, utilizing a single laser beam in a detonation-driven hypersonic shock tunnel. The Q1 branch of the NO A <- X (0, 0) transition was excited using radiation near 226.256 nm. It was determined that (1 + 1) REMPI of NO generates long lifetime fluorescence, and this observation is consistent with the findings reported by Jiang et al. [Opt. Lett. 49, 1297-1300 (2024)] and Leonov et al. [Opt. Lett. 49, 426-429 (2024)]. Single-shot tagged lines at microsecond delay times were obtained in a Ma6.9 hypersonic freestream flow using a long focal length lens. The measured average velocity of 3477 m/s agrees well with the simulation result.