中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction

文献类型:期刊论文

作者Dang, Shipei1,2; Qian, Jia2; Ma, Wang1,2; Ma, Rui1,2; Li, Xing1,2; Wang, Siying1,2; Bai, Chen2; Dan, Dan2; Yao, Baoli1,2
刊名PHOTONICS
出版日期2024-05
卷号11期号:5
关键词structured illumination microscopy optical sectioning full-color 3D reconstruction
ISSN号2304-6732
DOI10.3390/photonics11050405
产权排序1
英文摘要

The natural color of biological specimens plays a crucial role in body protection, signaling, physiological adaptations, etc. Full-color optical sectioning structured illumination microscopy (OS-SIM) color is a promising approach that can reconstruct biological specimens in three-dimension meanwhile maintaining their natural color. Full-color OS-SIM takes the advantages of rapid imaging speed, compatibility with fluorescence and non-fluorescence samples, compact configuration, and low cost. However, the commonly used HSV-RMS reconstruction algorithm for full-color OS-SIM faces two issues to be improved. One is the RMS (root-mean-square) OS reconstruction algorithm is prone to background noise, and the other is the reconstruction is bound in RGB and HSV color spaces, consuming more reconstructing time. In this paper, we propose a full-color Fourier-OS-SIM method that allows for the OS reconstruction using the high-frequency spectrum of the sample and thus is immune to the low-frequency background noise. The full-color Fourier-OS-SIM directly runs in the RGB color space, providing an easy way to restore the color information. Simulation and experiments with various samples (pollen grains and tiny animals) demonstrate that the full-color Fourier-OS-SIM method is superior to the HSV-RMS method regarding background noise suppression. Moreover, benefiting from the background noise suppression merit, the quantitative morphological height map analysis with the full-color Fourier-OS-SIM method is more accurate. The proposed full-color Fourier-OS-SIM method is expected to find broad applications in biological and industrial fields where the 3D morphology and the color information of objects both need to be recovered.

语种英语
WOS记录号WOS:001233232500001
出版者MDPI
源URL[http://ir.opt.ac.cn/handle/181661/97503]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Dan, Dan; Yao, Baoli
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
推荐引用方式
GB/T 7714
Dang, Shipei,Qian, Jia,Ma, Wang,et al. High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction[J]. PHOTONICS,2024,11(5).
APA Dang, Shipei.,Qian, Jia.,Ma, Wang.,Ma, Rui.,Li, Xing.,...&Yao, Baoli.(2024).High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction.PHOTONICS,11(5).
MLA Dang, Shipei,et al."High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction".PHOTONICS 11.5(2024).

入库方式: OAI收割

来源:西安光学精密机械研究所

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