Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders
文献类型：期刊论文
| 作者 | Lian, Luning4,5,6; Xing, Yi4,5,6; Zhang, Dayi2; Jiang, Longfei7; Song, Mengke1; Jiang, Bo3,4,5,6 |
| 刊名 | FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
 |
| 出版日期 | 2024 |
| 卷号 | 18期号:1页码:11 |
| 关键词 | Stable isotope probing (SIP) Magnetic-nanoparticle mediated isolation (MMI) Dimethoate Biodegradation Cultivation-independent approach |
| ISSN号 | 2095-2201 |
| DOI | 10.1007/s11783-024-1765-x |
| 英文摘要 | Microorganisms are crucial in the bioremediation of organophosphorus pesticides. However, most functional microorganisms (> 99%) are yet to be cultivated. This study applied two cultivation-independent approaches, DNA-SIP and magnetic-nanoparticle mediated isolation (MMI), to identify the functional microorganisms in degrading dimethoate in agricultural soils. MMI identified five dimethoate degraders: Pseudomonas, Bacillus, Ramlibacter, Arthrobacter, and Rhodococcus, whereas DNA-SIP identified three dimethoate degraders: Ramlibacter, Arthrobacter, and Rhodococcus. Also, MMI showed higher resolution than DNA-SIP in identifying functional microorganisms. Two organic phosphohydrolase (OPH) genes: ophC2 and ophB, were involved in dimethoate metabolism, as revealed by DNA-SIP and MMI. The degradation products of dimethoate include omethoate, O,O,S-trimethyl thiophosphorothioate, N-methyl-2-sulfanylacetamide, O,O-diethyl S-hydrogen phosphorodithioate, O,O,O-trimethyl thiophosphate, O,O,S-trimethyl thiophosphorodithioate, and O,O,O-trimethyl phosphoric. This study emphasizes the feasibility of using SIP and MMI to explore the functional dimethoate degraders, expanding our knowledge of microbial resources with cultivation-independent approaches. |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001049186200001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/74643]  |
| 专题 | 中国科学院广州地球化学研究所 |
| 通讯作者 | Xing, Yi; Jiang, Bo |
| 作者单位 | 1.South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Peoples R China 2.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China 3.Natl Engn Lab Site Remediat Technol, Beijing 100015, Peoples R China 4.Univ Sci & Technol Beijing, Sch Energy & Environm Engn, Beijing 100083, Peoples R China 5.Univ Sci & Technol Beijing, Natl Environm & Energy Sci & Technol Int Cooperat, Beijing 100083, Peoples R China 6.Univ Sci & Technol Beijing, Beijing Key Lab Resource oriented Treatment Ind Po, Beijing 100083, Peoples R China 7.Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lian, Luning,Xing, Yi,Zhang, Dayi,et al. Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING,2024,18(1):11. |
| APA | Lian, Luning,Xing, Yi,Zhang, Dayi,Jiang, Longfei,Song, Mengke,&Jiang, Bo.(2024).Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders.FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING,18(1),11. |
| MLA | Lian, Luning,et al."Comparative analysis of DNA-SIP and magnetic-nanoparticle mediated isolation (MMI) on unraveling dimethoate degraders".FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING 18.1(2024):11. |
入库方式： OAI收割
来源：广州地球化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。