Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells
文献类型：期刊论文
| 作者 | An, Jing2; Jiang, Jingjing2; Tang, Waner2; Zhong, Yufang2; Ren, Guofa2; Shang, Yu2; Yu, Zhiqiang1,3,4 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2023-09-01 |
| 卷号 | 262页码:10 |
| 关键词 | Triphenyl phosphate (TPHP) OH-TPHP DPHP Lipid metabolism PPARs signal pathway Endoplasmic reticulum stress |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2023.115160 |
| 英文摘要 | Triphenyl phosphate (TPHP) has been widely used as flame retardants and been detected with increasing frequency in environment. TPHP can transform into mono-hydroxylated phosphate (OH-TPHP) and diester diphenyl phosphate (DPHP) through biotransformation. So far, information on the cytotoxicity and molecular regulatory mechanisms of TPHP metabolites are still limit. This study investigated the adverse effects of TPHP, OH-TPHP, and DPHP in HepG2 cells in terms of cell proliferation, lactate dehydrogenase release, reactive oxygen species generation, and mitochondrial membrane potential. The transcriptomic changes were measured using RNA sequencing, and bioinformatics characteristics including biological functions, signal pathways and proteinprotein interaction were analyzed to explore the potential molecular mechanisms. Results displayed that the order of cytotoxicity was OH-TPHP> TPHP> DPHP. The prioritized biological functions changes induced by TPHP and OH-TPHP were correlated with lipid metabolism. Significant lipid accumulation was observed as confirmed by increased total cholesterol and triglycerides contents, and enhanced oil red O staining. Enrichment of PPAR & alpha;/& gamma; and down-stream genes suggested the participation of PPARs signal pathway in lipid metabolism disorder. In addition, TPHP and OH-TPHP induced endoplasmic reticulum stress (ERS), which was further confirmed by the ERS inhibitor experiment. In general, TPHP and OH-TPHP had obvious cytotoxic effects in HepG2 cells. PPARs signal pathway and endoplasmic reticulum stress may be involved in the lipid metabolism disorder induced by TPHP and OH-TPHP. |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:001032373900001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/74517]  |
| 专题 | 有机地球化学国家重点实验室 |
| 通讯作者 | Yu, Zhiqiang |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangdong Prov Key Lab Environm Protect & Resource, Guangzhou 510640, Peoples R China 2.Shanghai Univ, Inst Environm Pollut & Hlth, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China 3.CAS Ctr Excellence Deep Earth Sci, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Geochem, CAS Ctr Excellence Deep Earth Sci, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Jing,Jiang, Jingjing,Tang, Waner,et al. Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,262:10. |
| APA | An, Jing.,Jiang, Jingjing.,Tang, Waner.,Zhong, Yufang.,Ren, Guofa.,...&Yu, Zhiqiang.(2023).Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,262,10. |
| MLA | An, Jing,et al."Lipid metabolic disturbance induced by triphenyl phosphate and hydroxy metabolite in HepG2 cells".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 262(2023):10. |
入库方式： OAI收割
来源：广州地球化学研究所
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