Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC)
文献类型：期刊论文
| 作者 | Li, Jibing1,6,7; Luo, Chunling1,6,7 ; Cai, Xixi5; Dai, Yeliang1,6,7; Zhang, Dayi2,3,4; Zhang, Gan6,7
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| 刊名 | ENVIRONMENT INTERNATIONAL
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| 出版日期 | 2024-03-01 |
| 卷号 | 185页码:11 |
| 关键词 | Stable isotope probing Metagenome binning Cultivation Phenanthrene degrader Achromobacter |
| ISSN号 | 0160-4120 |
| DOI | 10.1016/j.envint.2024.108555 |
| 英文摘要 | High-throughput identification and cultivation of functional-yet-uncultivable microorganisms is a fundamental goal in environmental microbiology. It remains as a critical challenge due to the lack of routine and effective approaches. Here, we firstly proposed an approach of stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC) to isolate and characterize the active phenanthrene degraders from petroleumcontaminated soils. From SIP and metagenome, we assembled 13 high-quality metagenomic bins from 13C- DNA, and successfully obtained the genome of an active PHE degrader Achromobacter (genome-MB) from 13C- DNA metagenomes, which was confirmed by gyrB gene comparison and average nucleotide/amino identity (ANI/ AAI), as well as the quantification of PAH dioxygenase and antibiotic resistance genes. Thereinto, we modified the traditional cultivation medium with antibiotics and specific growth factors (e.g., vitamins and metals), and separated an active phenanthrene degrader Achromobacter sp. LJB-25 via directed isolation. Strain LJB-25 could degrade phenanthrene and its identity was confirmed by ANI/AAI values between its genome and genome-MB (>99 %). Our results hinted at the feasibility of SIP-MDC to identify, isolate and cultivate functional-yetuncultivable microorganisms (active phenanthrene degraders) from their natural habitats. Our findings developed a state-of-the-art SIP-MDC approach, expanded our knowledge on phenanthrene biodegradation mechanisms, and proposed a strategy to mine functional-yet-uncultivable microorganisms. |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001215840600001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/77050]  |
| 专题 | 有机地球化学国家重点实验室 |
| 通讯作者 | Zhang, Dayi |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Shenyang Univ, Key Lab Reg Environm & Ecorestorat, Minist Educ, Shenyang 110044, Peoples R China 3.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China 4.Jilin Univ, Key Lab Groundwater Resources & Environm, Minist Educ, Changchun 130021, Peoples R China 5.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China 6.Chinese Acad Sci, Guangdong Prov Key Lab Environm Protect & Resource, Guangzhou 510640, Peoples R China 7.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jibing,Luo, Chunling,Cai, Xixi,et al. Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC)[J]. ENVIRONMENT INTERNATIONAL,2024,185:11. |
| APA | Li, Jibing,Luo, Chunling,Cai, Xixi,Dai, Yeliang,Zhang, Dayi,&Zhang, Gan.(2024).Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC).ENVIRONMENT INTERNATIONAL,185,11. |
| MLA | Li, Jibing,et al."Cultivation and characterization of functional-yet-uncultivable phenanthrene degraders by stable-isotope-probing and metagenomic-binning directed cultivation (SIP-MDC)".ENVIRONMENT INTERNATIONAL 185(2024):11. |
入库方式： OAI收割
来源：广州地球化学研究所
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