SIP-metagenomics reveals key drivers of rhizospheric Benzo[a]pyrene bioremediation via bioaugmentation with indigenous soil microbes☆
文献类型：期刊论文
| 作者 | Zhao, Xuan1,4,6; Cheng, Xianghui2,4,6; Cai, Xixi3; Wang, Shuang4,5,6; Li, Jibing2,4,6; Dai, Yeliang4,6; Jiang, Longfei2,4,6; Luo, Chunling2,4,6 ; Zhang, Gan2,4,6
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| 刊名 | ENVIRONMENTAL POLLUTION
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| 出版日期 | 2024-11-01 |
| 卷号 | 360页码:12 |
| 关键词 | Stable isotope probing (SIP) Bioaugmentation Rhizoremediation benzo[a]pyrene degrader Metagenomic Indigenous soil microbes |
| ISSN号 | 0269-7491 |
| DOI | 10.1016/j.envpol.2024.124620 |
| 英文摘要 | Rhizoremediation and bioaugmentation have proven effective in promoting benzo[a]pyrene (BaP) degradation in contaminated soils. However, the mechanism underlying bioaugmented rhizospheric BaP degradation with native microbes is poorly understood. In this study, an indigenous BaP degrader (Stenotrophomonas BaP-1) isolated from petroleum-contaminated soil was introduced into ryegrass rhizosphere to investigate the relationship between indigenous degraders and rhizospheric BaP degradation. Stable isotope probing and 16S rRNA gene amplicon sequencing subsequently revealed 15 BaP degraders, 8 of which were directly associated with BaP degradation including Bradyrhizobium and Streptomyces. Bioaugmentation with strain BaP-1 significantly enhanced rhizospheric BaP degradation and shaped the microbial community structure. A correlation of BaP degraders, BaP degradation efficiency, and functional genes identified active degraders and genes encoding polycyclic aromatic hydrocarbon-ring hydroxylating dioxygenase (PAH-RHD) genes as the primary drivers of rhizospheric BaP degradation. Furthermore, strain BaP-1 was shown to not only engage in BaP metabolism but also to increase the abundance of other BaP degraders and PAH-RHD genes, resulting in enhanced rhizospheric BaP degradation. Metagenomic and correlation analyses indicated a significant positive relationship between glyoxylate and dicarboxylate metabolism and BaP degradation, suggesting a role for these pathways in rhizospheric BaP biodegradation. By identifying BaP degraders and characterizing their metabolic characteristics within intricate microbial communities, our study offers valuable insights into the mechanisms of bioaugmented rhizoremediation with indigenous bacteria for high-molecular-weight PAHs in petroleum-contaminated soils. |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001284398300001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/78231]  |
| 专题 | 有机地球化学国家重点实验室 |
| 通讯作者 | Li, Jibing |
| 作者单位 | 1.Kunming Univ, Coll Architecture & Civil Engn, Kunming 650214, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China 5.Chengdu Technol Univ, Sch Mat & Environm Engn, Chengdu 611730, Peoples R China 6.Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Hong Kong Macao Joint Lab Environm Pollu, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xuan,Cheng, Xianghui,Cai, Xixi,et al. SIP-metagenomics reveals key drivers of rhizospheric Benzo[a]pyrene bioremediation via bioaugmentation with indigenous soil microbes☆[J]. ENVIRONMENTAL POLLUTION,2024,360:12. |
| APA | Zhao, Xuan.,Cheng, Xianghui.,Cai, Xixi.,Wang, Shuang.,Li, Jibing.,...&Zhang, Gan.(2024).SIP-metagenomics reveals key drivers of rhizospheric Benzo[a]pyrene bioremediation via bioaugmentation with indigenous soil microbes☆.ENVIRONMENTAL POLLUTION,360,12. |
| MLA | Zhao, Xuan,et al."SIP-metagenomics reveals key drivers of rhizospheric Benzo[a]pyrene bioremediation via bioaugmentation with indigenous soil microbes☆".ENVIRONMENTAL POLLUTION 360(2024):12. |
入库方式： OAI收割
来源：广州地球化学研究所
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