Metagenomics and Stable Isotopes Uncover the Augmented Sulfide-Driven Autotrophic Denitrification in a Seasonally Hypoxic, Sulfate-Abundant Reservoir
文献类型：期刊论文
| 作者 | Yang, Mengdi3,4; Luo, Qianli3; Fan, Zhongya3; Zeng, Fantang3; Huang, Lu3; Ding, Shiyuan4,5; Cui, Gaoyang6; Li, Dongli4; Wei, Gangjian1,2; Liu, Cong-Qiang4,5 |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2024-07-31 |
| 卷号 | 58期号:32页码:14225-14236 |
| 关键词 | autotrophic denitrification sulfide oxidation dual isotopes metagenomicsequencing hypoxicreservoir |
| ISSN号 | 0013-936X |
| DOI | 10.1021/acs.est.4c00248 |
| 英文摘要 | The mechanism governing sulfur cycling in nitrate reduction within sulfate-rich reservoirs during seasonal hypoxic conditions remains poorly understood. This study employs nitrogen and oxygen isotope fractionation in nitrate, along with metagenomic sequencing to elucidate the intricacies of the coupled sulfur oxidation and nitrate reduction process in the water column. In the Aha reservoir, a typical seasonally stratified water body, we observed the coexistence of denitrification, bacterial sulfide oxidation, and bacterial sulfate reduction in hypoxic conditions. This is substantiated by the presence of abundant N/S-related genes (nosZ and aprAB/dsrAB) and fluctuations in N/S species. The lower (15)epsilon(NO3)/(18)epsilon(NO3) ratio (0.60) observed in this study, compared to heterotrophic denitrification, strongly supports the occurrence of sulfur-driven denitrification. Furthermore, we found a robust positive correlation between the metabolic potential of bacterial sulfide oxidation and denitrification (p < 0.05), emphasizing the role of sulfide produced via sulfate reduction in enhancing denitrification. Sulfide-driven denitrification relied on & sum;S2- as the primary electron donor preferentially oxidized by denitrification. The pivotal genus, Sulfuritalea, emerged as a central player in both denitrification and sulfide oxidation processes in hypoxic water bodies. Our study provides compelling evidence that sulfides assume a critical role in regulating denitrification in hypoxic water within an ecosystem where their contribution to the overall nitrogen cycle was previously underestimated. |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001282030900001 |
| 源URL | [http://ir.gig.ac.cn/handle/344008/78237]  |
| 专题 | 同位素地球化学国家重点实验室 |
| 通讯作者 | Li, Xiao-Dong |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Isotope Geochem, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Minist Ecol & Environm, South China Inst Environm Sci, State Environm Protect Key Lab Water Environm Simu, Guangzhou 510535, Peoples R China 4.Tianjin Univ, Inst Surface Earth Syst Sci, Sch Earth Syst Sci, Tianjin 300072, Peoples R China 5.Tianjin Univ, Tianjin Key Lab Earths Crit Zone Sci & Sustainable, Tianjin 300072, Peoples R China 6.Henan Univ, Coll Geog & Environm Sci, Kaifeng 475004, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Mengdi,Luo, Qianli,Fan, Zhongya,et al. Metagenomics and Stable Isotopes Uncover the Augmented Sulfide-Driven Autotrophic Denitrification in a Seasonally Hypoxic, Sulfate-Abundant Reservoir[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2024,58(32):14225-14236. |
| APA | Yang, Mengdi.,Luo, Qianli.,Fan, Zhongya.,Zeng, Fantang.,Huang, Lu.,...&Li, Xiao-Dong.(2024).Metagenomics and Stable Isotopes Uncover the Augmented Sulfide-Driven Autotrophic Denitrification in a Seasonally Hypoxic, Sulfate-Abundant Reservoir.ENVIRONMENTAL SCIENCE & TECHNOLOGY,58(32),14225-14236. |
| MLA | Yang, Mengdi,et al."Metagenomics and Stable Isotopes Uncover the Augmented Sulfide-Driven Autotrophic Denitrification in a Seasonally Hypoxic, Sulfate-Abundant Reservoir".ENVIRONMENTAL SCIENCE & TECHNOLOGY 58.32(2024):14225-14236. |
入库方式： OAI收割
来源：广州地球化学研究所
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