Word segmentation is crucial for reading in Chinese, where the absence of explicit word boundaries poses a distinct challenge. Previous studies in Chinese have examined how lexical and sub-lexical variables affect word segmentation. The present study investigated whether higher-level semantic information affects word segmentation using a primed word segmentation task with Overlapping Ambiguous Strings (OAS). An OAS is a three-character string in Chinese (e.g. ABC [in Latin letters]) where the middle character can constitute a word with both the left (word AB) and right (word BC) characters. The OAS was preceded by a semantic or repetition prime (presented for 42, 83, or 200 ms, across participants), priming either AB or BC. The semantic priming effect occurred at the 200-ms Stimulus Onset Asynchrony (SOA), whereas the repetition priming effect occurred at both 83 and 200-ms SOAs. These findings demonstrate that semantic information can affect word segmentation in Chinese within 200 ms.