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Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm

文献类型：期刊论文

作者Wang, Hao7,8; Xiao, Hongxiu6; Feng, Buhan6; Lan, Yi4,5; Fung, Cheuk Wang6; Zhang, Huan8; Yan, Guoyong4,5; Lian, Chao8; Zhong, Zhaoshan8; Li, Jing8
刊名SCIENCE ADVANCES
出版日期2024-07-24
卷号10期号:30页码:16
ISSN号2375-2548
DOI10.1126/sciadv.adn3053
通讯作者Wu, Angela Ruohao(angelawu@ust.hk) ; Li, Chaolun(lcl@qdio.ac.cn) ; Qian, Pei-Yuan(boqianpy@ust.hk)
英文摘要Vestimentiferan tubeworms that thrive in deep-sea chemosynthetic ecosystems rely on a single species of sulfide-oxidizing gammaproteobacterial endosymbionts housed in a specialized symbiotic organ called trophosome as their primary carbon source. While this simple symbiosis is remarkably productive, the host-symbiont molecular interactions remain unelucidated. Here, we applied an approach for deep-sea in situ single-cell fixation in a cold-seep tubeworm, Paraescarpia echinospica. Single-cell RNA sequencing analysis and further molecular characterizations of both the trophosome and endosymbiont indicate that the tubeworm maintains two distinct metabolic "microniches" in the trophosome by controlling the availability of chemosynthetic gases and metabolites, resulting in oxygenated and hypoxic conditions. The endosymbionts in the oxygenated niche actively conduct autotrophic carbon fixation and are digested for nutrients, while those in the hypoxic niche conduct anaerobic denitrification, which helps the host remove ammonia waste. Our study provides insights into the molecular interactions between animals and their symbiotic microbes.
WOS关键词WORM RIFTIA-PACHYPTILA ; SULFIDE BINDING ; PHYSIOLOGICAL PROTEOMICS ; CARBONIC-ANHYDRASE ; FORM-II ; INVERTEBRATE ; BLOOD ; LOCALIZATION ; SIBOGLINIDAE ; METHIONINE
资助项目Science and technology innovation Project of laoshan laboratory[lSKJ202203104] ; National natural Science Foundation of china[42030407] ; Guangdong Government[2019B030302004] ; Southern Marine Science and engineering Guangdong laboratory (Guangzhou)[2021hJ01] ; Southern Marine Science and engineering Guangdong laboratory (Guangzhou)[SMSeGl20Sc01] ; HKSAR government[16101822] ; HKSAR government[c2013- 22G] ; Chau Hoi Shuen Foundation
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001275948300024
出版者AMER ASSOC ADVANCEMENT SCIENCE
源URL[http://ir.qdio.ac.cn/handle/337002/186117]  
专题中国科学院海洋研究所
通讯作者Wu, Angela Ruohao; Li, Chaolun; Qian, Pei-Yuan
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Hong Kong, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China
6.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China
7.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Laoshan Lab, Qingdao, Peoples R China
8.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Hao,Xiao, Hongxiu,Feng, Buhan,et al. Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm[J]. SCIENCE ADVANCES,2024,10(30):16.
APA Wang, Hao.,Xiao, Hongxiu.,Feng, Buhan.,Lan, Yi.,Fung, Cheuk Wang.,...&Qian, Pei-Yuan.(2024).Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm.SCIENCE ADVANCES,10(30),16.
MLA Wang, Hao,et al."Single-cell RNA-seq reveals distinct metabolic "microniches" and close host-symbiont interactions in deep-sea chemosynthetic tubeworm".SCIENCE ADVANCES 10.30(2024):16.

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来源：海洋研究所

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